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In situ high-resolu...
In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum.
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- Nielsen, Jakob Toudahl (author)
- Center for Insoluble Protein Structures (inSPIN), and Interdisciplinary Nanoscience Center (iNANO) Department of Chemistry and Department of Chemistry, Aarhus C DK-8000, Denmark
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- Kulminskaya, Natalia V (author)
- Center for Insoluble Protein Structures (inSPIN), and Interdisciplinary Nanoscience Center (iNANO) Department of Chemistry and Department of Chemistry, Aarhus C DK-8000, Denmark
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- Bjerring, Morten (author)
- Center for Insoluble Protein Structures (inSPIN), and Interdisciplinary Nanoscience Center (iNANO) Department of Chemistry and Department of Chemistry, Aarhus C DK-8000, Denmark
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- Linnanto, Juha M (author)
- Institute of Physics, University of Tartu, Estonia
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- Rätsep, Margus (author)
- Institute of Physics, University of Tartu, Estonia
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- Pedersen, Marie Østergaard (author)
- Center for Insoluble Protein Structures (inSPIN), and Novo Nordisk a/s, Novo Nordisk Park, Denmark
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- Lambrev, Petar H (author)
- Hungarian Academy of Sciences, Biological Research Centre, Szeged, Hungary
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- Dorogi, Márta (author)
- Hungarian Academy of Sciences, Biological Research Centre, Szeged, Hungary
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- Garab, Győző (author)
- Hungarian Academy of Sciences, Biological Research Centre, Szeged, Hungary
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- Thomsen, Karen (author)
- Interdisciplinary Nanoscience Center (iNANO), Aarhus University
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- Jegerschöld, Caroline (author)
- Karolinska Institutet
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- Frigaard, Niels-Ulrik (author)
- Section for Marine Biology, Department of Biology, University of Copenhagen
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- Lindahl, Martin (author)
- Karolinska Institutet, Biovetenskaper och Nutrition, samt Strukturell Bioteknik, KTH
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- Nielsen, Niels Chr (author)
- Center for Insoluble Protein Structures (inSPIN), and Interdisciplinary Nanoscience Center (iNANO) Department of Chemistry and Department of Chemistry, Aarhus C DK-8000, Denmark
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Pedersen, MO (author)
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(creator_code:org_t)
- 2016-08-18
- 2016
- English.
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In: Nature Communications. - : Springer Science+Business Media B.V.. - 2041-1723. ; 7
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https://doi.org/10.1...
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Abstract
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- Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.
Keyword
- Biological Physics
- Biologisk fysik
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Nielsen, Jakob T ...
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Kulminskaya, Nat ...
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Bjerring, Morten
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Linnanto, Juha M
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Rätsep, Margus
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Pedersen, Marie ...
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show more...
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Lambrev, Petar H
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Dorogi, Márta
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Garab, Győző
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Thomsen, Karen
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Jegerschöld, Car ...
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Frigaard, Niels- ...
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Lindahl, Martin
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Nielsen, Niels C ...
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Pedersen, MO
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- Articles in the publication
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Nature Communica ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet