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Chemical characteri...
Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus
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- de Jesus, Liana Inara (author)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.
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- Smiderle, Fhernanda R. (author)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.
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- Ruthes, Andrea C. (author)
- KTH,Glykovetenskap
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- Vilaplana, Francisco, 1979- (author)
- KTH,Fiber- och polymerteknologi
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- Dal'Lin, Fernando Tonholi (author)
- Univ Fed Parana, Dept Pharmacol, CP 19046, Curitiba, PR, Brazil.
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- Maria-Ferreira, Daniele (author)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.;Univ Fed Parana, Dept Pharmacol, CP 19046, Curitiba, PR, Brazil.
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- Werner, Maria Fernanda (author)
- Univ Fed Parana, Dept Pharmacol, CP 19046, Curitiba, PR, Brazil.
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- Van Griensven, Leo J. L. D. (author)
- Wageningen Univ & Res, Plant Res Int, Bomsesteeg 1, NL-6708 PD Wageningen, Netherlands.
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- Iacomini, Marcello (author)
- Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil.
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Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil Glykovetenskap (creator_code:org_t)
- Elsevier, 2018
- 2018
- English.
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In: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 117, s. 1361-1366
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A water-soluble beta-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 x 10(5) Da with a random coil conformation for molecular weights below 1 x 10(6) Da. Typical signals of beta-(1 -> 3)-linkages were observed in NMR spectrum (delta 102.7/4.76; 102.8/4.74; 102.9/4.52; and delta 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at delta 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a beta-D-glucan with a main chain (1 -> 3)-linked, substituted at O-6 by single-units of glucose. The beta-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 mu g mL(-1) and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- beta-D-glucan
- Random coil
- Wound healing
Publication and Content Type
- ref (subject category)
- art (subject category)
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