Search: onr:"swepub:oai:DiVA.org:kth-235174" > Targeted Repression...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03346naa a2200445 4500 | |
001 | oai:DiVA.org:kth-235174 | |
003 | SwePub | |
008 | 180917s2018 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2351742 URI |
024 | 7 | a https://doi.org/10.1021/acssynbio.8b000562 DOI |
040 | a (SwePub)kth | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Shabestary, Kiyanu KTH,Systembiologi4 aut0 (Swepub:kth)u1a0fac0 |
245 | 1 0 | a Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria |
264 | c 2018-06-06 | |
264 | 1 | b American Chemical Society (ACS),c 2018 |
338 | a electronic2 rdacarrier | |
500 | a QC 20180920 | |
520 | a Photoautotrophic production of fuels and chemicals by cyanobacteria typically gives lower volumetric productivities and titers than heterotrophic production. Cyanobacteria cultures become light limited above an optimal cell density, so that this substrate is not supplied to all cells sufficiently. Here, we investigate genetic strategies for a two-phase cultivation, where biofuel-producing Synechocystis cultures are limited to an optimal cell density through inducible CRISPR interference (CRISPRi) repression of cell growth. Fixed CO2 is diverted to ethanol or n-butanol. Among the most successful strategies was partial repression of citrate synthase gltA. Strong repression (>90%) of gitA at low culture densities increased carbon partitioning to n-butanol 5-fold relative to a nonrepression strain, but sacrificed volumetric productivity due to severe growth restriction. CO2 fixation continued for at least 3 days after growth was arrested. By targeting sgRNAs to different regions of the gitA gene, we could modulate GItA expression and carbon partitioning between growth and product to increase both specific and volumetric productivity. These growth arrest strategies can be useful for improving performance of other photoautotrophic processes. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a cyanobacteria | |
653 | a CRISPRi | |
653 | a bioproduction | |
653 | a Bioteknologi | |
653 | a Biotechnology | |
700 | 1 | a Anfelt, Josefinu KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1035z36 |
700 | 1 | a Ljungqvist, Emilu KTH4 aut0 (Swepub:kth)u1qfloha |
700 | 1 | a Jahn, Michaelu KTH,Systembiologi4 aut0 (Swepub:kth)u1ryh6dw |
700 | 1 | a Yao, Lunu KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1nych9u |
700 | 1 | a Hudson, Elton P.u KTH,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1ja6ik3 |
710 | 2 | a KTHb Systembiologi4 org |
773 | 0 | t ACS Synthetic Biologyd : American Chemical Society (ACS)g 7:7q 7:7x 2161-5063 |
856 | 4 | u https://kth.diva-portal.org/smash/get/diva2:1248671/FULLTEXT01.pdfx primaryx Raw objecty fulltext:postprint |
856 | 4 | u http://kth.diva-portal.org/smash/get/diva2:1248671/FULLTEXT01 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-235174 |
856 | 4 8 | u https://doi.org/10.1021/acssynbio.8b00056 |
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