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Versatile gene-spec...
Versatile gene-specific sequence tags for Arabidopsis functional genomics : Trancript profiling and reverse genetics applications
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Hilson, P. (author)
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Allemeersch, J. (author)
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Altmann, T. (author)
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Aubourg, S. (author)
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Avon, A. (author)
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Beynon, J. (author)
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Bhalerao, R. P. (author)
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Bitton, F. (author)
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Caboche, M. (author)
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Cannoot, B. (author)
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Chardakov, V. (author)
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Cognet-Holliger, C. (author)
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Colot, V. (author)
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Crowe, M. (author)
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Darimont, C. (author)
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Durinck, S. (author)
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Eickhoff, H. (author)
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de Longevialle, A. F. (author)
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Farmer, E. E. (author)
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Grant, M. (author)
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Kuiper, M. T. R. (author)
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Lehrach, H. (author)
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Leon, C. (author)
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Leyva, A. (author)
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- Lundeberg, Joakim (author)
- KTH,Bioteknologi
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Lurin, C. (author)
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Moreau, Y. (author)
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Nietfeld, W. (author)
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Paz-Ares, J. (author)
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Reymond, P. (author)
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Rouze, P. (author)
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Sandberg, G. (author)
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Segura, M. D. (author)
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Serizet, C. (author)
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Tabrett, A. (author)
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Taconnat, L. (author)
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Thareau, V. (author)
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Van Hummelen, P. (author)
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Vercruysse, S. (author)
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Vuylsteke, M. (author)
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Weingartner, M. (author)
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Weisbeek, P. J. (author)
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- Wirta, Valtteri (author)
- KTH,Bioteknologi
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Wittink, F. R. A. (author)
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Zabeau, M. (author)
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Small, I. (author)
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(creator_code:org_t)
- 2004-10-15
- 2004
- English.
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In: Genome Research. - : Cold Spring Harbor Laboratory. - 1088-9051 .- 1549-5469. ; 14:10B, s. 2176-2189
- Related links:
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http://genome.cshlp....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- repeat-containing gene
- secondary cell-wall
- vacuolar h+-atpase
- microarray analysis
- escherichia-coli
- wide expression
- restorer gene
- plant-growth
- design
- thaliana
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Hilson, P.
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Allemeersch, J.
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Altmann, T.
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Aubourg, S.
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Avon, A.
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Beynon, J.
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show more...
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Bhalerao, R. P.
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Bitton, F.
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Caboche, M.
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Cannoot, B.
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Chardakov, V.
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Cognet-Holliger, ...
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Colot, V.
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Crowe, M.
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Darimont, C.
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Durinck, S.
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Eickhoff, H.
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de Longevialle, ...
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Farmer, E. E.
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Grant, M.
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Kuiper, M. T. R.
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Lehrach, H.
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Leon, C.
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Leyva, A.
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Lundeberg, Joaki ...
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Lurin, C.
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Moreau, Y.
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Nietfeld, W.
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Paz-Ares, J.
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Reymond, P.
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Rouze, P.
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Sandberg, G.
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Segura, M. D.
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Serizet, C.
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Tabrett, A.
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Taconnat, L.
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Thareau, V.
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Van Hummelen, P.
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Vercruysse, S.
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Vuylsteke, M.
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Weingartner, M.
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Weisbeek, P. J.
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Wirta, Valtteri
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Wittink, F. R. A ...
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Zabeau, M.
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Small, I.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Genome Research
- By the university
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Royal Institute of Technology