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- Hilson, P. (author)
Versatile gene-specific sequence tags for Arabidopsis functional genomics : Trancript profiling and reverse genetics applications
- Article/chapterEnglish2004
Publisher, publication year, extent ...
- 2004-10-15
- Cold Spring Harbor Laboratory,2004
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-23815
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-23815URI
- https://doi.org/10.1101/gr.2544504DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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- QC 20100525 QC 20110922
- Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- repeat-containing gene
- secondary cell-wall
- vacuolar h+-atpase
- microarray analysis
- escherichia-coli
- wide expression
- restorer gene
- plant-growth
- design
- thaliana
Added entries (persons, corporate bodies, meetings, titles ...)
- Allemeersch, J. (author)
- Altmann, T. (author)
- Aubourg, S. (author)
- Avon, A. (author)
- Beynon, J. (author)
- Bhalerao, R. P. (author)
- Bitton, F. (author)
- Caboche, M. (author)
- Cannoot, B. (author)
- Chardakov, V. (author)
- Cognet-Holliger, C. (author)
- Colot, V. (author)
- Crowe, M. (author)
- Darimont, C. (author)
- Durinck, S. (author)
- Eickhoff, H. (author)
- de Longevialle, A. F. (author)
- Farmer, E. E. (author)
- Grant, M. (author)
- Kuiper, M. T. R. (author)
- Lehrach, H. (author)
- Leon, C. (author)
- Leyva, A. (author)
- Lundeberg, JoakimKTH,Bioteknologi(Swepub:kth)u1qkn9kw (author)
- Lurin, C. (author)
- Moreau, Y. (author)
- Nietfeld, W. (author)
- Paz-Ares, J. (author)
- Reymond, P. (author)
- Rouze, P. (author)
- Sandberg, G. (author)
- Segura, M. D. (author)
- Serizet, C. (author)
- Tabrett, A. (author)
- Taconnat, L. (author)
- Thareau, V. (author)
- Van Hummelen, P. (author)
- Vercruysse, S. (author)
- Vuylsteke, M. (author)
- Weingartner, M. (author)
- Weisbeek, P. J. (author)
- Wirta, ValtteriKTH,Bioteknologi(Swepub:kth)u1u59vht (author)
- Wittink, F. R. A. (author)
- Zabeau, M. (author)
- Small, I. (author)
- KTHBioteknologi (creator_code:org_t)
Related titles
- In:Genome Research: Cold Spring Harbor Laboratory14:10B, s. 2176-21891088-90511549-5469
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- Bhalerao, R. P.
- Bitton, F.
- Caboche, M.
- Cannoot, B.
- Chardakov, V.
- Cognet-Holliger, ...
- Colot, V.
- Crowe, M.
- Darimont, C.
- Durinck, S.
- Eickhoff, H.
- de Longevialle, ...
- Farmer, E. E.
- Grant, M.
- Kuiper, M. T. R.
- Lehrach, H.
- Leon, C.
- Leyva, A.
- Lundeberg, Joaki ...
- Lurin, C.
- Moreau, Y.
- Nietfeld, W.
- Paz-Ares, J.
- Reymond, P.
- Rouze, P.
- Sandberg, G.
- Segura, M. D.
- Serizet, C.
- Tabrett, A.
- Taconnat, L.
- Thareau, V.
- Van Hummelen, P.
- Vercruysse, S.
- Vuylsteke, M.
- Weingartner, M.
- Weisbeek, P. J.
- Wirta, Valtteri
- Wittink, F. R. A ...
- Zabeau, M.
- Small, I.
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