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- Sjöberg, RonaldKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab (author)
High-density antigen microarrays for the assessment of antibody selectivity and off-target binding
- Article/chapterEnglish2018
Publisher, publication year, extent ...
- 2018-05-01
- New York, NY :Humana Press Inc.2018
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-238338
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-238338URI
- https://doi.org/10.1007/978-1-4939-7841-0_15DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
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Notes
- QC 20181115
- With the increasing availability of collections of antibodies, their evaluation in terms of binding selectivity becomes an important but challenging task. Planar antigen microarrays are very suitable tools to address this task and provide a powerful proteomics platform for the characterization of the binding selectivity of antibodies toward thousands of antigens in parallel. In this chapter, we describe our in-house developed procedures for the generation of high-density planar antigen microarrays with over 21,000 features. We also provide the details of the assay protocol, which we routinely use for the assessment of binding selectivity of the polyclonal antibodies generated within the Human Protein Atlas.
Subject headings and genre
- TEKNIK OCH TEKNOLOGIER Industriell bioteknik Annan industriell bioteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Industrial Biotechnology Other Industrial Biotechnology hsv//eng
- Affinity proteomics
- Antibody selectivity
- Antigen microarrays
- Protein microarrays
Added entries (persons, corporate bodies, meetings, titles ...)
- Andersson, EniKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u11gerbc (author)
- Hellström, CeciliaKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1doy1fn (author)
- Mattsson, CeciliaKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1adnwkp (author)
- Schwenk, Jochen M.KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1h7wtme (author)
- Nilsson, PeterKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1ws88sk (author)
- Ayoglu, BurcuKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1kfjubd (author)
- KTHAffinitets-proteomik (creator_code:org_t)
Related titles
- In:Epitope Mapping ProtocolsNew York, NY : Humana Press Inc., s. 231-2389781493978397
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