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Epitope mapping of ...
Epitope mapping of antibodies using bacterial cell surface display of gene fragment libraries
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- Volk, Anna-Luisa (author)
- KTH,Skolan för bioteknologi (BIO)
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- Hu, Francis Jingxin, 1986- (author)
- KTH,Skolan för kemi, bioteknologi och hälsa (CBH)
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- Rockberg, Johan (author)
- KTH,Skolan för kemi, bioteknologi och hälsa (CBH)
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(creator_code:org_t)
- 2018-05-01
- 2018
- English.
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In: Epitope Mapping Protocols. - New York, NY : Humana Press. ; , s. 141-157
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The unique property of specific high affinity binding to more or less any target of interest has made antibodies tremendously useful in numerous applications. Hence, knowledge of the precise binding site (epitope) of antibodies on the target protein is one of the most important features for understanding its performance and determining its reliability in immunoassays. Here, we describe an updated protocol for high-resolution method for mapping epitopes of antibodies based on bacterial surface expression of antigen fragments followed by antibody-based flow cytometric analysis. Epitopes are determined by DNA sequencing of the sorted antibody-binding cells followed by sequence alignment back to the antigen sequence. The method described here has been useful for the mapping of both monoclonal and polyclonal antibodies with varying sizes of epitopes.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Keyword
- Antibody
- Cell surface display
- Epitope mapping
- FACS
- Gram-positive
- S. carnosus
Publication and Content Type
- ref (subject category)
- kap (subject category)
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