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Protein engineering...
Protein engineering allows for mild affinity-based elution of therapeutic antibodies
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- Kanje, Sara, 1986- (author)
- KTH Royal Institute of Technology,KTH,Proteinteknologi,Hober lab
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- Venskutonytė, Raminta (author)
- Lund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups
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- Scheffel, Julia (author)
- KTH Royal Institute of Technology,KTH,Proteinteknologi,Hober lab
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- Nilvebrant, Johan, 1982- (author)
- KTH Royal Institute of Technology,KTH,Albanova VinnExcellence Center for Protein Technology, ProNova,Proteinvetenskap
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- Lindkvist-Petersson, Karin (author)
- Lund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups
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- Hober, Sophia, 1965- (author)
- KTH Royal Institute of Technology,KTH,Centrum för Bioprocessteknik, CBioPT,Science for Life Laboratory, SciLifeLab,Albanova VinnExcellence Center for Protein Technology, ProNova,Proteinteknologi
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(creator_code:org_t)
- Elsevier, 2018
- 2018
- English.
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In: Journal of Molecular Biology. - : Elsevier. - 0022-2836 .- 1089-8638. ; 430:18, s. 3427-3438
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https://doi.org/10.1...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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Abstract
Subject headings
Close
- Presented here is an engineered protein domain, based on Protein A, that displays a calcium-dependent binding to antibodies. This protein, ZCa, is shown to efficiently function as an affinity ligand for mild purification of antibodies through elution with ethylenediaminetetraacetic acid. Antibodies are commonly used tools in the area of biological sciences and as therapeutics, and the most commonly used approach for antibody purification is based on Protein A using acidic elution. Although this affinity-based method is robust and efficient, the requirement for low pH elution can be detrimental to the protein being purified. By introducing a calcium-binding loop in the Protein A-derived Z domain, it has been re-engineered to provide efficient antibody purification under mild conditions. Through comprehensive analyses of the domain as well as the ZCa–Fc complex, the features of this domain are well understood. This novel protein domain provides a very valuable tool for effective and gentle antibody and Fc-fusion protein purification
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Läkemedelsbioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Pharmaceutical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- antibody purification
- calcium-dependent binding
- Protein A
- protein engineering
- Z domain
- Bioteknologi
- Biotechnology
- antibody purification
- calcium-dependent binding
- Protein A
- protein engineering
- Z domain
Publication and Content Type
- ref (subject category)
- art (subject category)
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