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Single-particle ima...
Single-particle imaging without symmetry constraints at an X-ray free-electron laser
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- Rose, Max (author)
- DESY, Notkestr 85, D-22607 Hamburg, Germany.
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- Bobkov, Sergey (author)
- Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia.
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- Ayyer, Kartik (author)
- Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany.
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- Kurta, Ruslan P. (author)
- European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany.
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- Dzhigaev, Dmitry (author)
- DESY, Notkestr 85, D-22607 Hamburg, Germany.
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- Kim, Young Yong (author)
- DESY, Notkestr 85, D-22607 Hamburg, Germany.
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- Morgan, Andrew J. (author)
- Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany.
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- Yoon, Chun Hong (author)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA.
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- Westphal, Daniel (author)
- Uppsala universitet,Molekylär biofysik
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- Bielecki, Johan (author)
- Uppsala universitet,Molekylär biofysik,European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany
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- Sellberg, Jonas A. (author)
- Uppsala universitet,KTH,Biomedicinsk fysik och röntgenfysik,Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Uppsala, Sweden.;KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden.,Molekylär biofysik,SLAC Natl Accelerator Lab, Linac Coherent Light Source, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
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- Williams, Garth (author)
- Brookhaven Natl Lab, 98 Rochester St, Shirley, NY 11967 USA.
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- Maia, Filipe R.N.C. (author)
- Uppsala universitet,Molekylär biofysik,Lawrence Berkeley Natl Lab, NERSC, Berkeley, CA USA
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- Yefanov, Olexander M. (author)
- Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany.
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- Ilyin, Vyacheslav (author)
- Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia.
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- Mancuso, Adrian P. (author)
- European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany.
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- Chapman, Henry N. (author)
- Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany.
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- Hogue, Brenda G. (author)
- Arizona State Univ, Biodesign Inst, Biodesign Ctr Immunotherapy Vaccines & Virotherap, Tempe, AZ 85287 USA.;Arizona State Univ, Biodesign Inst, Biodesign Ctr Appl Struct Discovery, Tempe, AZ 85287 USA.;Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA.
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- Aquila, Andrew (author)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA.,DESY, Notkestr 85, D-22607 Hamburg, Germany;Natl Res Nucl Univ, MEPhI Moscow Engn Phys Inst, Kashirskoe Shosse 31, Moscow 115409, Russia
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- Barty, Anton (author)
- Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany.
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- Vartanyants, Ivan A. (author)
- DESY, Notkestr 85, D-22607 Hamburg, Germany.;Natl Res Nucl Univ, MEPhI Moscow Engn Phys Inst, Kashirskoe Shosse 31, Moscow 115409, Russia.
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DESY, Notkestr 85, D-22607 Hamburg, Germany Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia. (creator_code:org_t)
- INT UNION CRYSTALLOGRAPHY, 2018
- 2018
- English.
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In: IUCrJ. - : INT UNION CRYSTALLOGRAPHY. - 2052-2525. ; 5, s. 727-736
- Related links:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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Abstract
Subject headings
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- The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Keyword
- single-particle imaging
- three-dimensional virus reconstructions
- XFELs
Publication and Content Type
- ref (subject category)
- art (subject category)
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IUCrJ
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- By the author/editor
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Rose, Max
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Bobkov, Sergey
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Ayyer, Kartik
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Kurta, Ruslan P.
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Dzhigaev, Dmitry
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Kim, Young Yong
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show more...
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Morgan, Andrew J ...
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Yoon, Chun Hong
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Westphal, Daniel
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Bielecki, Johan
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Sellberg, Jonas ...
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Williams, Garth
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Maia, Filipe R.N ...
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Yefanov, Olexand ...
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Ilyin, Vyachesla ...
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Mancuso, Adrian ...
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Chapman, Henry N ...
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Hogue, Brenda G.
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Aquila, Andrew
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Barty, Anton
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Vartanyants, Iva ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Materials Chemis ...
- Articles in the publication
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IUCrJ
- By the university
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Royal Institute of Technology
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Uppsala University