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An electronically t...
An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy
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Dunsby, C. (author)
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Lanigan, P. M. P. (author)
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McGinty, J. (author)
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Elson, D. S. (author)
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Requejo-Isidro, J. (author)
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Munro, I. (author)
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Galletly, N. (author)
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McCann, F. (author)
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Treanor, B. (author)
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Önfelt, Björn (author)
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Davis, D. M. (author)
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Neil, M. A. A. (author)
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French, P. M. W. (author)
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- 2004-11-20
- 2004
- English.
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In: Journal of Physics D. - : IOP Publishing. - 0022-3727 .- 1361-6463. ; 37:23, s. 3296-3303
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Fluorescence imaging is used widely in microscopy and macroscopic imaging applications for fields ranging from biomedicine to materials science. A critical component for any fluorescence imaging system is the excitation source. Traditionally, wide-field systems use filtered thermal or arc-generated white light sources, while point scanning confocal microscope systems require spatially coherent (point-like) laser sources. Unfortunately, the limited range of visible wavelengths available from conventional laser sources constrains the design and usefulness of fluorescent probes in confocal microscopy. A 'hands-off' laser-like source, electronically tunable across the visible spectrum, would be invaluable for fluorescence imaging and provide new opportunities, e.g. automated excitation fingerprinting and in situ measurement of excitation cross-sections. Yet more information can be obtained using fluorescence lifetime imaging (FLIM), which requires that the light source be pulsed or rapidly modulated. We show how a white light continuum, generated by injecting femtosecond optical radiation into a micro-structured optical fibre, coupled with a simple prism-based tunable filter arrangement, can fulfil all these roles as a continuously electronically tunable (435-1150 nm) visible ultrafast light source in confocal, wide-field and FLIM systems.
Keyword
- photonic crystal fibers
- supercontinuum generation
- anomalous-dispersion
- continuum generation
- spatial-resolution
- raman-scattering
- optical-fiber
- multiphoton
- cell
- pulses
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dunsby, C.
-
Lanigan, P. M. P ...
-
McGinty, J.
-
Elson, D. S.
-
Requejo-Isidro, ...
-
Munro, I.
-
show more...
-
Galletly, N.
-
McCann, F.
-
Treanor, B.
-
Önfelt, Björn
-
Davis, D. M.
-
Neil, M. A. A.
-
French, P. M. W.
-
show less...
- Articles in the publication
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Journal of Physi ...
- By the university
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Royal Institute of Technology