An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy

Dunsby, C. (author)

Lanigan, P. M. P. (author)

McGinty, J. (author)

2004-11-20
2004
English.
In: Journal of Physics D. - : IOP Publishing. - 0022-3727 .- 1361-6463. ; 37:23, s. 3296-3303

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Fluorescence imaging is used widely in microscopy and macroscopic imaging applications for fields ranging from biomedicine to materials science. A critical component for any fluorescence imaging system is the excitation source. Traditionally, wide-field systems use filtered thermal or arc-generated white light sources, while point scanning confocal microscope systems require spatially coherent (point-like) laser sources. Unfortunately, the limited range of visible wavelengths available from conventional laser sources constrains the design and usefulness of fluorescent probes in confocal microscopy. A 'hands-off' laser-like source, electronically tunable across the visible spectrum, would be invaluable for fluorescence imaging and provide new opportunities, e.g. automated excitation fingerprinting and in situ measurement of excitation cross-sections. Yet more information can be obtained using fluorescence lifetime imaging (FLIM), which requires that the light source be pulsed or rapidly modulated. We show how a white light continuum, generated by injecting femtosecond optical radiation into a micro-structured optical fibre, coupled with a simple prism-based tunable filter arrangement, can fulfil all these roles as a continuously electronically tunable (435-1150 nm) visible ultrafast light source in confocal, wide-field and FLIM systems.

By the author/editor: Dunsby, C.; Lanigan, P. M. P ...; McGinty, J.; Elson, D. S.; Requejo-Isidro, ...; Munro, I.; show more...; Galletly, N.; McCann, F.; Treanor, B.; Önfelt, Björn; Davis, D. M.; Neil, M. A. A.; French, P. M. W.; show less...

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