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Using Scipion for stream image processing at Cryo-EM facilities

Gomez-Blanco, J. (author)
McGill Univ, Dept Anat & Cell Biol, Montreal, PQ, Canada.
de la Rosa-Trevín, José Miguel (author)
Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
Marabini, R. (author)
Univ Autonoma Madrid, Escuela Politecn Super, E-28049 Madrid, Spain.
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del Cano, L. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Jimenez, A. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Martinez, M. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Melero, R. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Majtner, T. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Maluenda, D. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Mota, J. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Rancel, Y. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Ramirez-Aportela, E. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Vilas, J. L. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Carroni, Marta (author)
Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
Fleischmann, Stefan (author)
Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
Lindahl, Erik, 1972- (author)
Stockholms universitet,KTH,SeRC - Swedish e-Science Research Centre,Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Stockholm, Sweden.;KTH Royal Inst Technol, Swedish E Sci Res Ctr, Stockholm, Sweden.,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),KTH Royal Institute of Technology, Sweden
Ashton, A. W. (author)
Harwell Sci & Innovat Campus, Diamond Light Source, Didcot OX11 0DE, Oxon, England.
Basham, M. (author)
Harwell Sci & Innovat Campus, Diamond Light Source, Didcot OX11 0DE, Oxon, England.
Clare, D. K. (author)
Harwell Sci & Innovat Campus, Diamond Light Source, Didcot OX11 0DE, Oxon, England.
Savage, K. (author)
Harwell Sci & Innovat Campus, Diamond Light Source, Didcot OX11 0DE, Oxon, England.
Siebert, C. A. (author)
Harwell Sci & Innovat Campus, Diamond Light Source, Didcot OX11 0DE, Oxon, England.
Sharov, G. G. (author)
MRC, Lab Mol Biol, Francis Crick Ave, Cambridge CB2 OQH, England.
Sorzano, C. O. S. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Conesa, P. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
Carazo, J. M. (author)
CSIC, Natl Ctr Biotechnol, Biocomp Unit, C Darwin 3,Campus Univ Autonoma, Madrid 28049, Spain.
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McGill Univ, Dept Anat & Cell Biol, Montreal, PQ, Canada Institutionen för biokemi och biofysik (creator_code:org_t)
ACADEMIC PRESS INC ELSEVIER SCIENCE, 2018
2018
English.
In: Journal of Structural Biology. - : ACADEMIC PRESS INC ELSEVIER SCIENCE. - 1047-8477 .- 1095-8657. ; 204:3, s. 457-463
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Three dimensional electron microscopy is becoming a very data-intensive field in which vast amounts of experimental images are acquired at high speed. To manage such large-scale projects, we had previously developed a modular workflow system called Scipion (de la Rosa-Trevfn et al., 2016). We present here a major extension of Scipion that allows processing of EM images while the data is being acquired. This approach helps to detect problems at early stages, saves computing time and provides users with a detailed evaluation of the data quality before the acquisition is finished. At present, Scipion has been deployed and is in production mode in seven Cryo-EM facilities throughout the world.

Subject headings

NATURVETENSKAP  -- Biologi -- Strukturbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Structural Biology (hsv//eng)
NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Keyword

Electron microscopy
Streaming Image processing
Live processing
High throughput
Scipion

Publication and Content Type

ref (subject category)
art (subject category)

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