Birth mode is associated with earliest strain-conferred gut microbiome functions and immunostimulatory potential

QC 20190626
The rate of caesarean section delivery (CSD) is increasing worldwide. It remains unclear whether disruption of mother-to-neonate transmission of microbiota through CSD occurs and whether it affects human physiology. Here we perform metagenomic analysis of earliest gut microbial community structures and functions. We identify differences in encoded functions between microbiomes of vaginally delivered (VD) and CSD neonates. Several functional pathways are over-represented in VD neonates, including lipopolysaccharide (LPS) biosynthesis. We link these enriched functions to individual-specific strains, which are transmitted from mothers to neonates in case of VD. The stimulation of primary human immune cells with LPS isolated from early stool samples of VD neonates results in higher levels of tumour necrosis factor (TNF-α) and interleukin 18 (IL-18). Accordingly, the observed levels of TNF-α and IL-18 in neonatal blood plasma are higher after VD. Taken together, our results support that CSD disrupts mother-to-neonate transmission of specific microbial strains, linked functional repertoires and immune-stimulatory potential during a critical window for neonatal immune system priming.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine hsv//eng
beta interferon
galectin 1
gamma interferon
granzyme B
interleukin 10
interleukin 12
interleukin 13
interleukin 15
interleukin 18
interleukin 1beta
interleukin 2
interleukin 21
interleukin 23
interleukin 23p40
interleukin 27
interleukin 4
interleukin 5
interleukin 6
interleukin 8
RNA 16S
tumor necrosis factor
unclassified drug
interleukin 18 protein
human
lipopolysaccharide
cell
community structure
digestive system
genetic analysis
immune system
microbial community
microorganism
neonate
physiology
amplicon
Article
bacterial strain
cesarean section
DNA sequence
female
gene function
genetic variability
human
human cell
human tissue
immunostimulation
intestine flora
marker gene
metagenomics
microbial colonization
newborn
quantitative analysis
real time polymerase chain reaction
sequence homology
taxonomy
vagina flora
vaginal delivery
genetics
in vitro study
metabolism
obstetric delivery
pregnancy
procedures
vertical transmission
Delivery
Obstetric
Gastrointestinal Microbiome
Humans
In Vitro Techniques
Infant
Newborn
Infectious Disease Transmission
Vertical
Interleukin-18
Lipopolysaccharides
Tumor Necrosis Factor-alpha

Heintz-Buschart, A. (author)
Fritz, J. V. (author)
Ramiro-Garcia, J. (author)
Habier, J. (author)
Herold, M. (author)
Narayanasamy, S. (author)
Kaysen, A. (author)
Hogan, A. H. (author)
Bindl, L. (author)
Bottu, J. (author)
Halder, R. (author)
Sjöqvist, ConnyKTH,Science for Life Laboratory, SciLifeLab(Swepub:kth)u19ca8zu (author)
May, P. (author)
Andersson, Anders F.KTH,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1hi776x (author)
de Beaufort, C. (author)
Wilmes, P. (author)
KTHScience for Life Laboratory, SciLifeLab (creator_code:org_t)

By the author/editor: Wampach, L.; Heintz-Buschart, ...; Fritz, J. V.; Ramiro-Garcia, J ...; Habier, J.; Herold, M.; show more...; Narayanasamy, S.; Kaysen, A.; Hogan, A. H.; Bindl, L.; Bottu, J.; Halder, R.; Sjöqvist, Conny; May, P.; Andersson, Ander ...; de Beaufort, C.; Wilmes, P.; show less...

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