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Using dextran of di...
Using dextran of different molecular weights to achieve faster eeze-drying and improved storage stability of lactate dehydrogenase
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- Larsen, Bjarke Strom (author)
- Univ Copenhagen, Dept Pharm, Copenhagen, Denmark. kytte, Jeppe
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Skytte, Jeppe (author)
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- Svagan, Anna Justina (author)
- KTH,Fiber- och polymerteknologi
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Meng-Lund, Helena (author)
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Grohganz, Holger (author)
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Löbmann, Korbinian (author)
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Univ Copenhagen, Dept Pharm, Copenhagen, Denmark kytte, Jeppe Fiber- och polymerteknologi (creator_code:org_t)
- 2018-06-20
- 2019
- English.
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In: Pharmaceutical development and technology (Print). - : Taylor & Francis. - 1083-7450 .- 1097-9867. ; 24:3, s. 323-328
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Freeze-drying of protein formulations is frequently used to maintain otein activity during storage. The freeze-drying process usually quires long primary drying times because the highest acceptable drying mperature to obtain acceptable products is dependent on the glass ansition temperature of the maximally freeze-concentrated solution -g). On the other hand, retaining protein activity during storage is lated to the glass transition temperature (T-g) of the final eeze-dried product. In this study, dextrans with different molecular ight (1 and 40kDa) and mixtures thereof at the ratio 3:1, 1:1, and 1:3 /w) were used as cryo-/lyoprotectant and their impact on the stability the model protein lactate dehydrogenase (LDH) was investigated at evated temperatures (40 degrees C and 60 degrees C). The dextran rmulations were then compared to formulations containing sucrose as yo-/lyoprotectant. Because of the higher T-g values of the dextrans, e primary drying times could be reduced compared to freeze-drying with crose. Similarly, the higher T-g and T-g of dextrans relative to crose led to benefits during storage which was shown through improved otection of LDH activity.
Subject headings
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
Keyword
- Dextran
- freeze-drying
- lactate dehydrogenase
- protein stability
- storage
Publication and Content Type
- ref (subject category)
- art (subject category)
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