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In-depth human plas...
In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta
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- Pernemalm, Maria (author)
- Karolinska Institutet
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- Sandberg, AnnSofi (author)
- Karolinska Institutet
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- Zhu, Yafeng (author)
- Karolinska Inst, Stockholm, Sweden.;Sci Life Lab, Proteogen, Solna, Sweden.
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- Boekel, Jorrit (author)
- Karolinska Institutet
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- Tamburro, Davide (author)
- Karolinska Inst, Stockholm, Sweden.
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- Schwenk, Jochen M. (author)
- KTH,Proteinvetenskap
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- Bjork, Albin (author)
- Karolinska Institutet
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- Wahren-Herlenius, Marie (author)
- Karolinska Institutet
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- Amark, Hanna (author)
- Karolinska Institutet
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- Ostenson, Claes-Goran (author)
- Karolinska Institutet
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- Westgren, Magnus (author)
- Karolinska Institutet
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- Lehtio, Janne (author)
- Karolinska Institutet
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Karolinska Institutet Karolinska Inst, Stockholm, Sweden;Sci Life Lab, Proteogen, Solna, Sweden. (creator_code:org_t)
- ELIFE SCIENCES PUBLICATIONS LTD, 2019
- 2019
- English.
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In: eLIFE. - : ELIFE SCIENCES PUBLICATIONS LTD. - 2050-084X. ; 8
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.7...
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Abstract
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- Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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eLIFE
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- By the author/editor
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Pernemalm, Maria
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Sandberg, AnnSof ...
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Zhu, Yafeng
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Boekel, Jorrit
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Tamburro, Davide
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Schwenk, Jochen ...
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show more...
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Bjork, Albin
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Wahren-Herlenius ...
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Amark, Hanna
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Ostenson, Claes- ...
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Westgren, Magnus
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Lehtio, Janne
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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eLIFE
- By the university
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Royal Institute of Technology
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Karolinska Institutet