Search: onr:"swepub:oai:DiVA.org:kth-255741" >
Systematic Developm...
Systematic Development of Sandwich Immunoassays for the Plasma Secretome
-
- Häussler, Ragna S. (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Bendes, Annika (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Iglesias, Maria Jesus (author)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Division of Internal Medicine, University Hospital of North Norway, Tromsø, 9010, Norway
-
show more...
-
- Sanchez-Rivera, Laura (author)
- KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab
-
- Dodig-Crnkovic, Tea (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Byström, Sanna (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Fredolini, Claudia (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Birgersson, Elin (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Dale, Matilda (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Edfors, Fredrik (author)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
-
- Fagerberg, Linn (author)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
-
- Rockberg, Johan (author)
- KTH,Proteinteknologi
-
- Tegel, Hanna (author)
- KTH,Proteinteknologi
-
- Uhlèn, Mathias (author)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab,Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, 2970, Denmark
-
- Qundos, Ulrika (author)
- Atlas Antibodies AB, Bromma, 168 69, Sweden
-
- Schwenk, Jochen M. (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
show less...
-
(creator_code:org_t)
- 2019-07-22
- 2019
- English.
-
In: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861.
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://onlinelibrar...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- The plasma proteome offers a clinically useful window into human health. Recent advances from highly multiplexed assays now call for appropriate pipelines to validate individual candidates. Here, a workflow is developed to build dual binder sandwich immunoassays (SIA) and for proteins predicted to be secreted into plasma. Utilizing suspension bead arrays, ≈1800 unique antibody pairs are first screened against 209 proteins with recombinant proteins as well as EDTA plasma. Employing 624 unique antibodies, dilution-dependent curves in plasma and concentration-dependent curves of full-length proteins for 102 (49%) of the targets are obtained. For 22 protein assays, the longitudinal, interindividual, and technical performance is determined in a set of plasma samples collected from 18 healthy subjects every third month over 1 year. Finally, 14 of these assays are compared with with SIAs composed of other binders, proximity extension assays, and affinity-free targeted mass spectrometry. The workflow provides a multiplexed approach to screen for SIA pairs that suggests using at least three antibodies per target. This design is applicable for a wider range of targets of the plasma proteome, and the assays can be applied for discovery but also to validate emerging candidates derived from other platforms.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- antibodies
- plasma
- sandwich assays
- screening
- secreted proteins
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Proteomics
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Häussler, Ragna ...
-
Bendes, Annika
-
Iglesias, Maria ...
-
Sanchez-Rivera, ...
-
Dodig-Crnkovic, ...
-
Byström, Sanna
-
show more...
-
Fredolini, Claud ...
-
Birgersson, Elin
-
Dale, Matilda
-
Edfors, Fredrik
-
Fagerberg, Linn
-
Rockberg, Johan
-
Tegel, Hanna
-
Uhlèn, Mathias
-
Qundos, Ulrika
-
Schwenk, Jochen ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
- Articles in the publication
-
Proteomics
- By the university
-
Royal Institute of Technology