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Carbamyl adducts on...
Carbamyl adducts on low-density lipoprotein induce IgG response in LDLR-/- mice and bind plasma autoantibodies in humans under enhanced carbamylation.
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Kummu, Outi (author)
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Turunen, S Pauliina (author)
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Wang, Chunguang (author)
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Lehtimäki, Jaakko (author)
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Veneskoski, Marja (author)
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Kastarinen, Helena (author)
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Koivula, Marja-Kaisa (author)
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Risteli, Juha (author)
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Kesäniemi, Y Antero (author)
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Hörkkö, Sohvi (author)
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- Mary Ann Liebert Inc, 2013
- 2013
- English.
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In: Antioxidants and Redox Signaling. - : Mary Ann Liebert Inc. - 1523-0864 .- 1557-7716. ; 19:10, s. 1047-62
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- AIMS: Post-translational modification of proteins via carbamylation predicts increased risk for coronary artery disease. Uremia and smoke exposure are known to increase carbamylation. The aim was to investigate the role of carbamylated low-density lipoprotein (LDL) immunization on antibody formation and atherogenesis in LDL receptor-deficient (LDLR-/-) mice, and to study autoantibodies to carbamylated proteins in humans with carbamylative load.RESULTS: LDLR-/- mice immunized with carbamylated mouse LDL (msLDL; n=10) without adjuvant showed specific immunoglobulin G (IgG) antibody levels to carbamyl-LDL and malondialdehyde-modified LDL (MDA-LDL) but not to oxidized LDL or native LDL. Immunization did not influence the atherosclerotic plaque area compared with control LDLR-/- mice immunized with native msLDL (n=10) or phosphate-buffered saline (n=11). Humans with high plasma urea levels, as well as smokers, had increased IgG autoantibody levels to carbamyl-modified proteins compared to the subjects with normal plasma urea levels, or to nonsmokers.INNOVATION: Carbamyl-LDL induced specific IgG antibody response cross-reactive with MDA-LDL in mice. IgG antibodies to carbamyl-LDL were also found in human plasma and related to conditions known to have increased carbamylation, such as uremia and smoking. Plasma antibodies to carbamylated proteins may serve as new indicator of in vivo carbamylation.CONCLUSION: These data give insight into mechanisms of in vivo humoral recognition of post-translationally modified structures. Humoral IgG immune response to carbamylated proteins is suggested to play a role in conditions leading to enhanced carbamylation, such as uremia and smoking.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Kummu, Outi
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Turunen, S Pauli ...
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Wang, Chunguang
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Lehtimäki, Jaakk ...
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Veneskoski, Marj ...
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Kastarinen, Hele ...
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show more...
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Koivula, Marja-K ...
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Risteli, Juha
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Kesäniemi, Y Ant ...
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Hörkkö, Sohvi
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
- Articles in the publication
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Antioxidants and ...
- By the university
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Royal Institute of Technology