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Aldehyde-alcohol de...
Aldehyde-alcohol dehydrogenase forms a high-order spirosome architecture critical for its activity
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- Kim, Gijeong (author)
- Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea.
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- Azmi, Liyana (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Glasgow G12 8QQ, Lanark, Scotland.
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- Jang, Seongmin (author)
- Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea.
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- Jung, Taeyang (author)
- KTH,Strukturell bioteknik,Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea.
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- Hebert, Hans (author)
- KTH,Strukturell bioteknik,Karolinska Inst, Dept Biosci & Nutr, S-14183 Huddinge, Sweden.
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- Roe, Andrew J. (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Glasgow G12 8QQ, Lanark, Scotland.
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- Byron, Olwyn (author)
- Univ Glasgow, Sch Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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- Song, Ji-Joon (author)
- Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea.
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Korea Adv Inst Sci & Technol, Dept Biol Sci, Daejeon 34141, South Korea Univ Glasgow, Inst Infect Immun & Inflammat, Glasgow G12 8QQ, Lanark, Scotland. (creator_code:org_t)
- 2019-10-04
- 2019
- English.
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In: Nature Communications. - : Nature Publishing Group. - 2041-1723. ; 10:1
- Related links:
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Aldehyde-alcohol dehydrogenase (AdhE) is a key enzyme in bacterial fermentation, converting acetyl-CoA to ethanol, via two consecutive catalytic reactions. Here, we present a 3.5 angstrom resolution cryo-EM structure of full-length AdhE revealing a high-order spirosome architecture. The structure shows that the aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH) active sites reside at the outer surface and the inner surface of the spirosome respectively, thus topologically separating these two activities. Furthermore, mutations disrupting the helical structure abrogate enzymatic activity, implying that formation of the spirosome structure is critical for AdhE activity. In addition, we show that this spirosome structure undergoes conformational change in the presence of cofactors. This work presents the atomic resolution structure of AdhE and suggests that the high-order helical structure regulates its enzymatic activity.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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