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  • Lorenzen, EmilyRockefeller Univ, Lab Chem Biol & Signal Transduct, 1230 York Ave, New York, NY 10065 USA. (author)

Multiplexed analysis of the secretin-like GPCR-RAMP interactome

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • AMER ASSOC ADVANCEMENT SCIENCE,2019
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-263373
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-263373URI
  • https://doi.org/10.1126/sciadv.aaw2778DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:142118179URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20191115
  • Receptor activity-modifying proteins (RAMPs) have been shown to modulate the functions of several G protein-coupled receptors (GPCRs), but potential direct interactions among the three known RAMPs and hundreds of GPCRs have never been investigated. Focusing mainly on the secretin-like family of GPCRs, we engineered epitope-tagged GPCRs and RAMPs, and developed a multiplexed suspension bead array (SBA) immunoassay to detect GPCR-RAMP complexes from detergent-solubilized lysates. Using 64 antibodies raised against the native proteins and 4 antibodies targeting the epitope tags, we mapped the interactions among 23 GPCRs and 3 RAMPs. We validated nearly all previously reported secretin-like GPCR-RAMP interactions, and also found previously unidentified RAMP interactions with additional secretin-like GPCRs, chemokine receptors, and orphan receptors. The results provide a complete interactome of secretin-like GPCRs with RAMPs. The SBA strategy will be useful to search for additional GPCR-RAMP complexes and other interacting membrane protein pairs in cell lines and tissues.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Dodig-Crnkovic, TeaKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1wrth1x (author)
  • Kotliar, Ilana B.Rockefeller Univ, Lab Chem Biol & Signal Transduct, 1230 York Ave, New York, NY 10065 USA.;Tri Inst PhD Program Chem Biol, New York, NY 10065 USA. (author)
  • Pin, ElisaKTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1pmjyje (author)
  • Ceraudo, EmilieRockefeller Univ, Lab Chem Biol & Signal Transduct, 1230 York Ave, New York, NY 10065 USA. (author)
  • Vaughan, Roger D.Rockefeller Univ, Ctr Clin & Translat Sci, 1230 York Ave, New York, NY 10065 USA. (author)
  • Uhlén, MathiasKTH,Science for Life Laboratory, SciLifeLab,Systembiologi(Swepub:kth)u1dulvmw (author)
  • Huber, ThomasRockefeller Univ, Lab Chem Biol & Signal Transduct, 1230 York Ave, New York, NY 10065 USA. (author)
  • Schwenk, Jochen M.KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1h7wtme (author)
  • Sakmar, Thomas P.Karolinska Institutet (author)
  • Rockefeller Univ, Lab Chem Biol & Signal Transduct, 1230 York Ave, New York, NY 10065 USA.Affinitets-proteomik (creator_code:org_t)

Related titles

  • In:Science Advances: AMER ASSOC ADVANCEMENT SCIENCE5:92375-2548

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