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TcellSubC :
TcellSubC : An Atlas of the Subcellular Proteome of Human T Cells
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- Joshi, Rubin Narayan (author)
- Karolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden.
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- Stadler, Charlotte (author)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
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- Lehmann, Robert (author)
- King Abdullah Univ Sci & Technol, Comp Elect & Math Sci & Engn Div, Biol & Environm Sci & Engn Div, Thuwal, Saudi Arabia.
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- Lehtio, Janne (author)
- Karolinska Institutet
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- Tegner, Jesper (author)
- Karolinska Institutet
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- Schmidt, Angelika (author)
- Karolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden.;Ludwig Maximilians Univ Munchen, Inst Immunol, Biomed Ctr, Munich, Germany.
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- Vesterlund, Mattias (author)
- Karolinska Institutet
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Karolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden. Science for Life Laboratory, SciLifeLab (creator_code:org_t)
- 2019-11-26
- 2019
- English.
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In: Frontiers in Immunology. - : FRONTIERS MEDIA SA. - 1664-3224. ; 10
- Related links:
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https://urn.kb.se/re...
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Abstract
Subject headings
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- We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- subcellular fractionation
- subcellular localization
- CD4 T cells
- TCR stimulation
- protein translocation
- mass spectrometry-based proteomics
Publication and Content Type
- ref (subject category)
- art (subject category)
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