TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells

Joshi, Rubin NarayanKarolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden. (author)

TcellSubC : An Atlas of the Subcellular Proteome of Human T Cells

QC 20200107
We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.

Stadler, CharlotteKTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap(Swepub:kth)u1r100ik (author)
Lehmann, RobertKing Abdullah Univ Sci & Technol, Comp Elect & Math Sci & Engn Div, Biol & Environm Sci & Engn Div, Thuwal, Saudi Arabia. (author)
Lehtio, JanneKarolinska Institutet (author)
Tegner, JesperKarolinska Institutet (author)
Schmidt, AngelikaKarolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden.;Ludwig Maximilians Univ Munchen, Inst Immunol, Biomed Ctr, Munich, Germany. (author)
Vesterlund, MattiasKarolinska Institutet (author)
Karolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden.Science for Life Laboratory, SciLifeLab (creator_code:org_t)

By the author/editor: Joshi, Rubin Nar ...; Stadler, Charlot ...; Lehmann, Robert; Lehtio, Janne; Tegner, Jesper; Schmidt, Angelik ...; show more...; Vesterlund, Matt ...; show less...

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