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TcellSubC :
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Joshi, Rubin NarayanKarolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden.
(author)
TcellSubC : An Atlas of the Subcellular Proteome of Human T Cells
- Article/chapterEnglish2019
Publisher, publication year, extent ...
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2019-11-26
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FRONTIERS MEDIA SA,2019
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-266307
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-266307URI
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https://doi.org/10.3389/fimmu.2019.02708DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:142437077URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20200107
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We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Stadler, CharlotteKTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap(Swepub:kth)u1r100ik
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Lehmann, RobertKing Abdullah Univ Sci & Technol, Comp Elect & Math Sci & Engn Div, Biol & Environm Sci & Engn Div, Thuwal, Saudi Arabia.
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Lehtio, JanneKarolinska Institutet
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Tegner, JesperKarolinska Institutet
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Schmidt, AngelikaKarolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden.;Ludwig Maximilians Univ Munchen, Inst Immunol, Biomed Ctr, Munich, Germany.
(author)
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Vesterlund, MattiasKarolinska Institutet
(author)
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Karolinska Inst, Ctr Mol Med, Dept Med Solna, Unit Computat Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Sci Life Lab, Stockholm, Sweden.Science for Life Laboratory, SciLifeLab
(creator_code:org_t)
Related titles
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In:Frontiers in Immunology: FRONTIERS MEDIA SA101664-3224
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