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Robotic fluidic cou...
Robotic fluidic coupling and interrogation of multiple vascularized organ chips
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Novak, R. (author)
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Ingram, M. (author)
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Marquez, S. (author)
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show more...
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Das, D. (author)
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Delahanty, A. (author)
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- Herland, Anna (author)
- Karolinska Institutet,KTH,Mikro- och nanosystemteknik
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Maoz, B. M. (author)
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Jeanty, S. S. F. (author)
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Somayaji, M. R. (author)
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Burt, M. (author)
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Calamari, E. (author)
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Chalkiadaki, A. (author)
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Cho, A. (author)
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Choe, Y. (author)
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Chou, D. B. (author)
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Cronce, M. (author)
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Dauth, S. (author)
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Divic, T. (author)
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Fernandez-Alcon, J. (author)
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Ferrante, T. (author)
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Ferrier, J. (author)
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FitzGerald, E. A. (author)
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Fleming, R. (author)
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Jalili-Firoozinezhad, S. (author)
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Grevesse, T. (author)
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Goss, J. A. (author)
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Hamkins-Indik, T. (author)
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Henry, O. (author)
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Hinojosa, C. (author)
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Huffstater, T. (author)
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Jang, K. -J (author)
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Kujala, V. (author)
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Leng, L. (author)
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Mannix, R. (author)
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Milton, Y. (author)
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Nawroth, J. (author)
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Nestor, B. A. (author)
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Ng, C. F. (author)
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O’Connor, B. (author)
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Park, T. -E (author)
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Sanchez, H. (author)
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Sliz, J. (author)
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Sontheimer-Phelps, A. (author)
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Swenor, B. (author)
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Thompson, G. , I I (author)
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Touloumes, G. J. (author)
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Tranchemontagne, Z. (author)
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Wen, N. (author)
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Yadid, M. (author)
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Bahinski, A. (author)
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Hamilton, G. A. (author)
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Levner, D. (author)
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Levy, O. (author)
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Przekwas, A. (author)
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Prantil-Baun, R. (author)
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Parker, K. K. (author)
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Ingber, D. E. (author)
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(creator_code:org_t)
- 2020-01-27
- 2020
- English.
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In: Nature Biomedical Engineering. - : Nature Research. - 2157-846X.
- Related links:
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https://www.ncbi.nlm...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Subject headings
Close
- Organ chips can recapitulate organ-level (patho)physiology, yet pharmacokinetic and pharmacodynamic analyses require multi-organ systems linked by vascular perfusion. Here, we describe an ‘interrogator’ that employs liquid-handling robotics, custom software and an integrated mobile microscope for the automated culture, perfusion, medium addition, fluidic linking, sample collection and in situ microscopy imaging of up to ten organ chips inside a standard tissue-culture incubator. The robotic interrogator maintained the viability and organ-specific functions of eight vascularized, two-channel organ chips (intestine, liver, kidney, heart, lung, skin, blood–brain barrier and brain) for 3 weeks in culture when intermittently fluidically coupled via a common blood substitute through their reservoirs of medium and endothelium-lined vascular channels. We used the robotic interrogator and a physiological multicompartmental reduced-order model of the experimental system to quantitatively predict the distribution of an inulin tracer perfused through the multi-organ human-body-on-chips. The automated culture system enables the imaging of cells in the organ chips and the repeated sampling of both the vascular and interstitial compartments without compromising fluidic coupling.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Keyword
- Blood
- Physiological models
- Tissue culture
- Culture systems
- Experimental system
- In-situ microscopies
- Liquid handling
- Organ systems
- Reduced order models
- Sample collection
- Vascular perfusion
- Robotics
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Novak, R.
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Ingram, M.
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Marquez, S.
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Das, D.
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Delahanty, A.
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Herland, Anna
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show more...
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Maoz, B. M.
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Jeanty, S. S. F.
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Somayaji, M. R.
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Burt, M.
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Calamari, E.
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Chalkiadaki, A.
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Cho, A.
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Choe, Y.
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Chou, D. B.
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Cronce, M.
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Dauth, S.
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Divic, T.
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Fernandez-Alcon, ...
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Ferrante, T.
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Ferrier, J.
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FitzGerald, E. A ...
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Fleming, R.
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Jalili-Firoozine ...
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Grevesse, T.
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Goss, J. A.
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Hamkins-Indik, T ...
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Henry, O.
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Hinojosa, C.
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Huffstater, T.
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Jang, K. -J
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Kujala, V.
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Leng, L.
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Mannix, R.
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Milton, Y.
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Nawroth, J.
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Nestor, B. A.
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Ng, C. F.
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O’Connor, B.
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Park, T. -E
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Sanchez, H.
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Sliz, J.
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Sontheimer-Phelp ...
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Swenor, B.
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Thompson, G. , I ...
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Touloumes, G. J.
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Tranchemontagne, ...
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Wen, N.
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Yadid, M.
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Bahinski, A.
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Hamilton, G. A.
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Levner, D.
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Levy, O.
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Przekwas, A.
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Prantil-Baun, R.
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Parker, K. K.
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Ingber, D. E.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
- Articles in the publication
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Nature Biomedica ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet