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Biological Laborato...
Biological Laboratory X-Ray Microscopy
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- Kördel, Mikael (author)
- KTH,Biomedicinsk fysik och röntgenfysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden
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- Fogelqvist, Emelie (author)
- KTH,Biomedicinsk fysik och röntgenfysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden
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- Carannante, Valentina (author)
- Karolinska Inst, Dept Microbiol Tumor & Cell Biol, S-17177 Solna, Sweden.
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- Önfelt, Björn (author)
- KTH,Biomedicinsk fysik och röntgenfysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biophys, S-10691 Stockholm, Sweden
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- Reddy, Hemanth K.N. (author)
- Uppsala universitet,Molekylär biofysik
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- Svenda, Martin (author)
- Uppsala universitet,Molekylär biofysik
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- Sellberg, Jonas A. (author)
- KTH,Biomedicinsk fysik och röntgenfysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden
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- Arsana, Komang G.Y. (author)
- KTH,Biomedicinsk fysik och röntgenfysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden
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- Vogt, Ulrich (author)
- KTH,Biomedicinsk fysik och röntgenfysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden
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- Hertz, Hans (author)
- KTH,Biomedicinsk fysik och röntgenfysik,KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden
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(creator_code:org_t)
- SPIE - International Society for Optical Engineering, 2019
- 2019
- English.
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In: X-Ray Nanoimaging. - : SPIE - International Society for Optical Engineering. - 9781510629189
- Related links:
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http://kth.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Zone-plate-based soft x-ray microscopes operating in the water window allow high-resolution and high-contrast imaging of intact cells in their near-native state. Laboratory-source-based x-ray microscopes are an important complement to the accelerator-based instruments, providing high accessibility and allowing close integration with other cell-biological techniques. Here we present recent biological applications using the Stockholm laboratory water-window x-ray microscope, which is based on a liquid-nitrogen-jet laser-plasma source. Technical improvements to the microscope in the last few years have resulted in increased x-ray flux at the sample and significantly improved stability and reliability. In addition to this, vibrations in key components have been measured, analyzed and reduced to improve the resolution to 25 nm half-period. The biological applications include monitoring the development of carbon-dense vesicles in starving human embryonic kidney cells (HEK293T), imaging the interaction between natural killer (NK) cells and HEK293T target cells, and most recently studying a newly discovered giant DNA virus and the process of viral replication inside a host amoeba. All biological imaging was done on cryo-frozen hydrated samples in 2D and in some cases 3D.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- X-ray microscopy
- water-window
- laboratory
- resolution
- cell imaging
- cryo
Publication and Content Type
- ref (subject category)
- kon (subject category)
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To the university's database
- By the author/editor
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Kördel, Mikael
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Fogelqvist, Emel ...
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Carannante, Vale ...
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Önfelt, Björn
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Reddy, Hemanth K ...
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Svenda, Martin
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show more...
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Sellberg, Jonas ...
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Arsana, Komang G ...
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Vogt, Ulrich
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Hertz, Hans
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
- Articles in the publication
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X-Ray Nanoimagin ...
- By the university
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Royal Institute of Technology
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Uppsala University