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Immunoassays on thi...
Immunoassays on thiol-ene synthetic paper generate a superior fluorescence signal
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- Guo, Weijin (author)
- KTH,Mikro- och nanosystemteknik,Microfluidics
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- Vilaplana, Lluisa (author)
- IQAC-CSIC
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- Hansson, Jonas (author)
- KTH,Mikro- och nanosystemteknik,Microfluidics
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- Marco, M.-Pilar (author)
- IQAC-CSIC
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- van der Wijngaart, Wouter, 1973- (author)
- KTH,Mikro- och nanosystemteknik,Microfluidics
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(creator_code:org_t)
- Elsevier, 2020
- 2020
- English.
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In: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 163
- Related links:
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https://kth.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- The fluorescence-based detection of biological complexes on solid substrates is widely used in microarrays and lateral flow tests. Here, we investigate thiol-ene micropillar scaffold sheets (“synthetic paper”) as the solid substrate in such assays. Compared to state-of-the-art glass and nitrocellulose substrates, assays on synthetic paper provide a stronger fluorescence signal, similar or better reproducibility, lower limit of detection (LOD), and the possibility of working with lower immunoreagent concentrations. Using synthetic paper, we detected the antibiotic enrofloxacin in whole milk with a LOD of 1.64 nM, which is on par or better than the values obtained with other common tests, and much lower than the maximum level allowed by European Union regulations. The significance of these results lays in that they indicate that synthetically-derived microstructured substrate materials have the potential to improve the performance of diagnostic assays.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Polymerteknologi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Polymer Technologies (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Pappers-, massa- och fiberteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Paper, Pulp and Fiber Technology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Maskinteknik -- Strömningsmekanik och akustik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Mechanical Engineering -- Fluid Mechanics and Acoustics (hsv//eng)
Keyword
- Biosensor; Enrofloxacin; Fluorescence; Glass slides; Microarray; Nitrocellulose; Off-stoichiometric thiol-ene; Porous substrate
- Bioteknologi
- Biotechnology
- Electrical Engineering
- Elektro- och systemteknik
- Kemi
- Chemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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