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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005904naa a2200709 4500
001oai:DiVA.org:kth-278779
003SwePub
008200729s2020 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2787792 URI
0247 a https://doi.org/10.1016/j.nbt.2020.05.0022 DOI
040 a (SwePub)kth
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Tegel, Hannau KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1nou8yx
2451 0a High throughput generation of a resource of the human secretome in mammalian cells
264 1b Elsevier BV,c 2020
338 a print2 rdacarrier
500 a QC 20200729
520 a The proteins secreted by human tissues and blood cells, the secretome, are important both for the basic understanding of human biology and for identification of potential targets for future diagnosis and therapy. Here, a high-throughput mammalian cell factory is presented that was established to create a resource of recombinant full-length proteins covering the majority of those annotated as 'secreted' in humans. The full-length DNA sequences of each of the predicted secreted proteins were generated by gene synthesis, the constructs were transfected into Chinese hamster ovary (CHO) cells and the recombinant proteins were produced, purified and analyzed. Almost 1,300 proteins were successfully generated and proteins predicted to be secreted into the blood were produced with a success rate of 65%, while the success rates for the other categories of secreted proteins were somewhat lower giving an overall one-pass success rate of ca. 58%. The proteins were used to generate targeted proteomics assays and several of the proteins were shown to be active in a phenotypic assay involving pancreatic beta-cell dedifferentiation. Many of the proteins that failed during production in CHO cells could be rescued in human embryonic kidney (HEK 293) cells suggesting that a cell factory of human origin can be an attractive alternative for production in mammalian cells. In conclusion, a high-throughput protein production and purification system has been successfully established to create a unique resource of the human secretome.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
653 a secreted proteins
653 a protein production
653 a protein puri fication
653 a high-throughput
7001 a Dannemeyer, Melanieu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1u0d39z
7001 a Kanje, Sara,d 1986-u KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1t204cn
7001 a Sivertsson, Åsau KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u195d5b0
7001 a Berling, Annau KTH,Proteinteknologi4 aut0 (Swepub:kth)u18q4b67
7001 a Svensson, Anne-Sophieu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u1t0mckt
7001 a Hober, Andreasu KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u15bkxsl
7001 a Enstedt, Henricu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1svlatx
7001 a Volk, Anna-Luisau KTH,Proteinteknologi4 aut0 (Swepub:kth)u1j049o2
7001 a Lundqvist, Magnusu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1hfekeg
7001 a Moradi, Mona4 aut
7001 a Afshari, Delaramu KTH,Proteinteknologi4 aut0 (Swepub:kth)u18nn4qt
7001 a Ekblad, Siriu KTH,Proteinvetenskap4 aut0 (Swepub:kth)u13gulih
7001 a Xu, LanLanu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1qu1kuf
7001 a Vestin, Malinu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1qp5jk2
7001 a Bidad, Faranaku KTH,Proteinteknologi4 aut0 (Swepub:kth)u13gbknj
7001 a Schiavone, Lovisa Holmbergu AstraZeneca, R&D, Discovery Biol, Discovery Sci, Gothenburg, Sweden.4 aut
7001 a Davies, Ricku AstraZeneca, R&D, Discovery Biol, Discovery Sci, Cambridge, England.4 aut
7001 a Mayr, Lorenz M.u Syncona Investment Ltd, London, England.;Swiss Fed Inst Technol, B BIOL, Mol Hlth Sci, Zurich, Switzerland.4 aut
7001 a Knight, Sineadu AstraZeneca, R&D, Discovery Biol, Discovery Sci, Cambridge, England.4 aut
7001 a Gopel, Sven O.u AstraZeneca, BioPharmaceut R&D, Biosci Metab Res & Early Dev Cardiovasc Renal & M, Gothenburg, Sweden.4 aut
7001 a Voldborg, Bjorn G.u Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Lyngby, Denmark.4 aut
7001 a Edfors, Fredriku KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u149ml0e
7001 a Forsström, Björnu KTH,Science for Life Laboratory, SciLifeLab,Proteinteknologi4 aut0 (Swepub:kth)u11i5nju
7001 a von Feilitzen, Kalleu KTH,Proteinteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dbmi38
7001 a Zwahlen, Martinu KTH,Science for Life Laboratory, SciLifeLab,Proteinteknologi4 aut0 (Swepub:kth)u1fcjpxe
7001 a Rockberg, Johanu KTH,Proteinteknologi4 aut0 (Swepub:kth)u130jo0a
7001 a Takanen, Jenny Ottossonu KTH,Proteinteknologi4 aut0 (Swepub:kth)u1lthxtb
7001 a Uhlén, Mathiasu KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1dulvmw
7001 a Hober, Sophia,d 1965-u KTH,Proteinteknologi4 aut0 (Swepub:kth)u11qqzc1
7102 a KTHb Proteinvetenskap4 org
7730 t New Biotechnologyd : Elsevier BVg 58, s. 45-54q 58<45-54x 1871-6784x 1876-4347
8564 u https://doi.org/10.1016/j.nbt.2020.05.002y Fulltext
8564 u https://doi.org/10.1016/j.nbt.2020.05.002
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-278779
8564 8u https://doi.org/10.1016/j.nbt.2020.05.002

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