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Recombinant Spider ...
Recombinant Spider Silk Forms Tough and Elastic Nanomembranes that are Protein‐Permeable and Support Cell Attachment and Growth
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- Gustafsson, Linnea (author)
- KTH,Mikro- och nanosystemteknik
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- Panagiotis Tasiopoulos, Christos (author)
- KTH,Proteinteknologi,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Jansson, Ronnie (author)
- KTH,Proteinvetenskap,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Kvick, Mathias (author)
- Spiber technologies AB
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- Duursma, Thijs (author)
- KTH,Mikro- och nanosystemteknik
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- Gasser, T. Christian (author)
- KTH,Hållfasthetslära
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- van der Wijngaart, Wouter (author)
- KTH,Mikro- och nanosystemteknik
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- Hedhammar, My (author)
- KTH,Proteinteknologi,Albanova VinnExcellence Center for Protein Technology, ProNova
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(creator_code:org_t)
- 2020-08-16
- 2020
- English.
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In: Advanced Functional Materials. - Stockholm : Wiley. - 1616-301X .- 1616-3028. ; 30:40, s. 2002982-
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Abstract
Subject headings
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- Biologically compatible membranes are of high interest for several biological and medical applications. Tissue engineering, for example, would greatly benefit from ultrathin, yet easy‐to‐handle, biodegradable membranes that are permeable to proteins and support cell growth. In this work, nanomembranes are formed by self‐assembly of a recombinant spider silk protein into a nanofibrillar network at the interface of a standing aqueous solution. The membranes are cm‐sized, free‐standing, bioactive and as thin as 250 nm. Despite their nanoscale thickness, the membranes feature an ultimate engineering strain of over 220% and a toughness of 5.2 MPa. Moreover, they are permeable to human blood plasma proteins and promote cell adherence and proliferation. Human keratinocytes seeded on either side of the membrane form a confluent monolayer within three days. The significance of these results lays in the unique combination of nanoscale thickness, elasticity, toughness, biodegradability, protein permeability and support for cell growth, as this may enable new applications in tissue engineering including bi‐layered in vitro tissue models and support for clinical transplantation of coherent cell layers.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- recombinant spider silk
- nanomembranes
- elasticity
- permeability
- tissue engineering
Publication and Content Type
- ref (subject category)
- art (subject category)
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