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Nuclear Receptor Bi...
Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression
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- Xiong, Anqi, 1986- (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden.
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- Roy, Ananya (author)
- Uppsala universitet,Neuroonkologi,Science for Life Laboratory, SciLifeLab
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- Spyrou, Argyris (author)
- Uppsala universitet,KTH,Mikro- och nanosystemteknik,Uppsala Univ, Dept Immunol Genet & Pathol, Sci Life Lab, S-75185 Uppsala, Sweden.,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,KTH Royal Inst Technol, Dept Intelligent Syst, S-10044 Stockholm, Sweden.
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- Weishaupt, Holger (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Neuroonkologi
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- Marinescu, Voichita (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi
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- Olofsson, Tommie (author)
- Natl Board Forens Med, S-75237 Uppsala, Sweden.
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- Hermanson, Ola (author)
- Karolinska Institutet
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- Swartling, Fredrik J. (author)
- Uppsala Univ, Dept Immunol Genet & Pathol, Sci Life Lab, S-75185 Uppsala, Sweden.
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- Forsberg Nilsson, Karin, 1963- (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Neuroonkologi
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- Johansson, Fredrik K., 1975- (author)
- Uppsala universitet,Neuroonkologi,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2020-06-06
- 2020
- English.
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In: Cancers. - : MDPI AG. - 2072-6694. ; 12:6
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Subject headings
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- Pseudokinases, comprising 10% of the human kinome, are emerging as regulators of canonical kinases and their functions are starting to be defined. We previously identified the pseudokinase Nuclear Receptor Binding Protein 2 (NRBP2) in a screen for genes regulated during neural differentiation. During mouse brain development,NRBP2is expressed in the cerebellum, and in the adult brain, mainly confined to specific neuronal populations. To study the role of NRBP2 in brain tumors, we stained a brain tumor tissue array for NRPB2, and find its expression to be low, or absent, in a majority of the tumors. This includes medulloblastoma (MB), a pediatric tumor of the cerebellum. Using database mining of published MB data sets, we also find that NRBP2 is expressed at a lower level in MB than in the normal cerebellum. Recent studies indicate that MB exhibits frequent epigenetic alternations and we therefore treated MB cell lines with drugs inhibiting DNA methylation or histone deacetylation, which leads to an upregulation of NRBP2 mRNA expression, showing that it is under epigenetic regulation in cultured MB cells. Furthermore, forced overexpression of NRBP2 in MB cell lines causes a dramatic decrease in cell numbers, increased cell death, impaired cell migration and inhibited cell invasion in vitro. Taken together, our data indicate that downregulation of NRBP2 may be a feature by which MB cells escape growth regulation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- brain tumor
- pseudokinase
- NRBP
- pediatric cancer
- apoptosis
Publication and Content Type
- ref (subject category)
- art (subject category)
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Cancers
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Xiong, Anqi, 198 ...
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Roy, Ananya
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Spyrou, Argyris
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Weishaupt, Holge ...
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Marinescu, Voich ...
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Olofsson, Tommie
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Hermanson, Ola
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Swartling, Fredr ...
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Forsberg Nilsson ...
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Johansson, Fredr ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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Cancers
- By the university
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Royal Institute of Technology
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Uppsala University
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Karolinska Institutet