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Transient state microscopy : a new tool for biomolecular imaging

Sanden, Tor (author)
KTH,Experimentell biomolekylär fysik
Persson, Gustav (author)
KTH,Experimentell biomolekylär fysik
Widengren, Jerker (author)
KTH,Experimentell biomolekylär fysik
 (creator_code:org_t)
Bellingham, WA : SPIE-INT SOC OPTICAL ENGINEERING, 2009
2009
English.
In: MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES IX. - Bellingham, WA : SPIE-INT SOC OPTICAL ENGINEERING. - 9780819474292
  • Conference paper (peer-reviewed)
Abstract Subject headings
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  • Photoinduced transient dark states are exhibited by practically all common fluorophores. However, their information content has to date only been sparsely exploited due to methodological constraints. Here, a new concept is presented and verified that can monitor and image these states via their photodynamic fingerprints. It unites the environmental sensitivity of these states with the sensitivity of fluorescence-based detection. For demonstration, triplet state images of liposomes in different environments were generated, showing how local environmental differences can be resolved, not clearly distinguishable via other fluorescence parameters. The concept can provide several new, useful and independent fluorescence-based parameters in biomolecular imaging.

Subject headings

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

modulated excitation
laser scanning microscopy
transient state imaging
triplet state imaging
oxygen monitoring
Analytical chemistry
Analytisk kemi

Publication and Content Type

ref (subject category)
kon (subject category)

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Persson, Gustav
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