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Influence of Membra...
Influence of Membrane Phase on the Optical Properties of DPH
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- Osella, Silvio (author)
- Chemical and Biological Systems Simulation Lab, Centre of New Technologies, University of Warsaw, Banacha 2c, 02-097 Warsaw, Poland
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- Paloncyova, Marketa (author)
- KTH,Teoretisk kemi och biologi,Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic
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- Sahi, Maryam (author)
- KTH,Teoretisk kemi och biologi
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- Knippenberg, Stefan (author)
- KTH,Teoretisk kemi och biologi,Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic; Theory Lab, Hasselt University, Agoralaan Building D, 3590 Diepenbeek, Belgium
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(creator_code:org_t)
- 2020-09-17
- 2020
- English.
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In: Molecules. - : MDPI AG. - 1431-5157 .- 1420-3049. ; 25:18
- Related links:
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https://doi.org/10.3...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- The fluorescent molecule diphenylhexatriene (DPH) has been often used in combination with fluorescence anisotropy measurements, yet little is known regarding the non-linear optical properties. In the current work, we focus on them and extend the application to fluorescence, while paying attention to the conformational versatility of DPH when it is embedded in different membrane phases. Extensive hybrid quantum mechanics/molecular mechanics calculations were performed to investigate the influence of the phase- and temperature-dependent lipid environment on the probe. Already, the transition dipole moments and one-photon absorption spectra obtained in the liquid ordered mixture of sphingomyelin (SM)-cholesterol (Chol) (2:1) differ largely from the ones calculated in the liquid disordered DOPC and solid gel DPPC membranes. Throughout the work, the molecular conformation in SM:Chol is found to differ from the other environments. The two-photon absorption spectra and the ones obtained by hyper-Rayleigh scattering depend strongly on the environment. Finally, a stringent comparison of the fluorescence anisotropy decay and the fluorescence lifetime confirm the use of DPH to gain information upon the surrounding lipids and lipid phases. DPH might thus open the possibility to detect and analyze different biological environments based on its absorption and emission properties.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Keyword
- conformationally versatile molecules
- QM
- MM
- absorption
- photoselection
- fluorescence decay
- fluorescence anisotropy
- hyper-Rayleigh scattering
- two-photon absorption
Publication and Content Type
- ref (subject category)
- art (subject category)
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