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Transcriptome studi...
Transcriptome studies of the early cone-setting acrocona mutant provide evidence for a functional conservation of the age-dependent flowering pathway between angiosperms and gymnosperms.
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- Akhter, Shirin (author)
- Sveriges Lantbruksuniversitet
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- Westrin, Karl Johan, 1989- (author)
- KTH,Genteknologi,Emanuelsson Lab
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- Zivi, Nathan (author)
- Sveriges lantbruksuniversitet, Skogforsk
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- Nordal, Veronika (author)
- Sveriges Lantbruksuniversitet
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- Kretzschmar, Warren W. (author)
- Karolinska Institutet
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- Delhomme, Nicolas (author)
- Sveriges Lantbruksuniversitet
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- Street, Nathaniel R. (author)
- Umeå Universitet
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- Nilsson, Ove (author)
- Sveriges Lantbruksuniversitet
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- Emanuelsson, Olof (author)
- KTH,Genteknologi,Emanuelsson Lab
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- Sundström, Jens (author)
- Sveriges lantbruksuniversitet
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- All seed plants go through a juvenile period before they initiate seed- and pollen-bearing organs and reproduce. Reproductive phase-change is well characterized in angiosperm model species, but much less well described in gymnosperms. Here, we utilize the early cone-setting acrocona mutant to study reproductive phase change in the conifer Picea abies; a representative of the gymnosperm lineage. The acrocona mutant frequently initiates cone-like structures, called transition shoots, in positions where wild-type P. abies always produces vegetative shoots. By sequence analysis of mRNA and microRNA transcripts, we demonstrate that orthologous components of the Age-dependent flowering pathway are active at the time of cone initiation. We show that a member of the SQUAMOSA BINDING PROTEIN-LIKE (SPL) gene family, PaSPL7, is active in reproductive meristems, whereas a putative negative regulator of PaSPL7, microRNA156 is upregulated in vegetative meristem. By allele-specific assembly, we also identify a short nucleotide polymorphism (SNP) in the miRNA156 binding of PaSPL7. By genotyping a segregating population of inbred acrocona trees, we show a clear co-segregation between the early cone-setting phenotype and trees homozygous for the SNP. Hence, the data presented demonstrate evolutionary conservation of the age-dependent flowering pathway and involvement of this pathway in regulating cone-setting in the conifer P. abies.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- Bioteknologi
- Biotechnology
Publication and Content Type
- vet (subject category)
- ovr (subject category)
To the university's database
- By the author/editor
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Akhter, Shirin
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Westrin, Karl Jo ...
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Zivi, Nathan
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Nordal, Veronika
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Kretzschmar, War ...
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Delhomme, Nicola ...
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show more...
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Street, Nathanie ...
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Nilsson, Ove
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Emanuelsson, Olo ...
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Sundström, Jens
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
- By the university
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Royal Institute of Technology