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Production of β-car...
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Kim, Jung HunDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
(author)
Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures
- Article/chapterEnglish2009
Publisher, publication year, extent ...
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2009-11-05
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Springer Science and Business Media LLC,2009
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-297825
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-297825URI
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https://doi.org/10.1007/s12257-008-0230-1DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20210824
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Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37°C were 1.7-fold and 2-fold higher, respectively, than those at 28°C. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Kim, Seon WonDivision of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea
(author)
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Nguyen, Do Quynh AnhDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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Li, He,D.Sc,1985-Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea(Swepub:kth)u192ayff
(author)
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Kim, Sung BaeDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
(author)
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Seo, Yang GonDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
(author)
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Yang, Jae KyungDivision of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea
(author)
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Chung, In YoungDepartment of Electronic and Communications Engineering, Kwangwoon University, Seoul, 139-701, Korea
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Kim, Dae HwanSchool of Electrical Engineering, Kookmin University, Seoul, 136-702, Korea
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Kim, Chang JoonDepartment of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
(author)
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Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, KoreaDivision of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea
(creator_code:org_t)
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In:Biotechnology and Bioprocess Engineering: Springer Science and Business Media LLC14:5, s. 559-5641226-83721976-3816
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Kim, Jung Hun
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Kim, Seon Won
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Nguyen, Do Quynh ...
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Li, He, D.Sc, 19 ...
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Kim, Sung Bae
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Seo, Yang Gon
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Yang, Jae Kyung
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Chung, In Young
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Kim, Dae Hwan
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Kim, Chang Joon
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