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Production of β-car...
Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures
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- Kim, Jung Hun (author)
- Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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- Kim, Seon Won (author)
- Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea
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- Nguyen, Do Quynh Anh (author)
- Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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- Li, He, D.Sc, 1985- (author)
- Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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- Kim, Sung Bae (author)
- Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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- Seo, Yang Gon (author)
- Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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- Yang, Jae Kyung (author)
- Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju, 660-701, Korea
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- Chung, In Young (author)
- Department of Electronic and Communications Engineering, Kwangwoon University, Seoul, 139-701, Korea
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- Kim, Dae Hwan (author)
- School of Electrical Engineering, Kookmin University, Seoul, 136-702, Korea
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- Kim, Chang Joon (author)
- Department of Chemical & Biological Engineering and ERI, Gyeongsang National University, Jinju, 660-701, Korea
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(creator_code:org_t)
- 2009-11-05
- 2009
- English.
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In: Biotechnology and Bioprocess Engineering. - : Springer Science and Business Media LLC. - 1226-8372 .- 1976-3816. ; 14:5, s. 559-564
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https://link.springe...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37°C were 1.7-fold and 2-fold higher, respectively, than those at 28°C. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Kim, Jung Hun
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Kim, Seon Won
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Nguyen, Do Quynh ...
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Li, He, D.Sc, 19 ...
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Kim, Sung Bae
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Seo, Yang Gon
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Yang, Jae Kyung
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Chung, In Young
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Kim, Dae Hwan
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Kim, Chang Joon
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Bioprocess Techn ...
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Biotechnology an ...
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Royal Institute of Technology