Woven Electroanalytical Biosensor for Nucleic AcidAmplification Tests

• Fiber-based biosensors enable a new approach in analytical diagnosticdevices. The majority of textile-based biosensors, however, rely oncolorimetric detection. Here a woven biosensor that integrates microfluidicsstructures in combination with an electroanalytical readout based on athiol-self-assembled monolayer (SAM) for Nucleic Acid Amplification Testing,NAATs is shown. Two types of fiber-based electrodes are systematicallycharacterized: pure gold microwires (bond wire) and off-the-shelf plasmagold-coated polyester multifilament threads to evaluate their potential to formSAMs on their surface and their electrochemical performance in woven textile.A woven electrochemical DNA (E-DNA) sensor using a SAM-based stem-loopprobe-modified gold microwire is fabricated. These sensors can specificallydetect unpurified, isothermally amplified genomic DNA of Staphylococcusepidermidis (10 copies/μL) by recombinase polymerase amplification (RPA).This work demonstrates that textile-based biosensors have the potential forintegrating and being employed as automated, sample-to-answer analyticaldevices for point-of-care (POC) diagnostics.

Subject headings

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

Woven electroanalytical microfluidic devices

DNA biosensors

Fiber electrodes

Self assembled monolayers (SAM)

Publication and Content Type

ref (subject category)

art (subject category)

kfu (subject category)