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Spatial mapping rev...
Spatial mapping reveals human adipocyte subpopulations with distinct sensitivities to insulin
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- Baeckdahl, Jesper (author)
- Karolinska Institutet
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- Franzén, Lovisa (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Massier, Lucas (author)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med H7, C2-94, S-14186 Stockholm, Sweden.
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- Li, Qian (author)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med H7, C2-94, S-14186 Stockholm, Sweden.
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- Jalkanen, Jutta (author)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med H7, C2-94, S-14186 Stockholm, Sweden.
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- Gao, Hui (author)
- Karolinska Institutet
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- Andersson, Alma (author)
- Karolinska Institutet,KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Bhalla, Nayanika (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Thorell, Anders (author)
- Karolinska Inst, Danderyd Hosp, Ersta Hosp, Dept Clin Sci, S-11691 Stockholm, Sweden.;Karolinska Inst, Dept Surg, Ersta Hosp, S-11691 Stockholm, Sweden.
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- Ryden, Mikael (author)
- Karolinska Institutet
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- Ståhl, Patrik, Dr. (author)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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- Mejhert, Niklas (author)
- Karolinska Institutet
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(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English.
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In: Cell Metabolism. - : Elsevier BV. - 1550-4131 .- 1932-7420. ; 33:9, s. 1869-
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Abstract
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- The contribution of cellular heterogeneity and architecture to white adipose tissue (WAT) function is poorly understood. Herein, we combined spatially resolved transcriptional profiling with single-cell RNA sequencing and image analyses to map human WAT composition and structure. This identified 18 cell classes with unique propensities to form spatially organized homo-and heterotypic clusters. Of these, three constituted mature adipocytes that were similar in size, but distinct in their spatial arrangements and transcriptional profiles. Based on marker genes, we termed these Adipo(LEP), Adipo(PLIN), and Adipo(SAA). We confirmed, in independent datasets, that their respective gene profiles associated differently with both adipocyte and whole-body insulin sensitivity. Corroborating our observations, insulin stimulation in vivo by hyperinsulinemic-euglycemic clamp showed that only Adipo(PLIN) displayed a transcriptional response to insulin. Altogether, by mining this multimodal resource we identify that human WAT is composed of three classes of mature adipocytes, only one of which is insulin responsive.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Baeckdahl, Jespe ...
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Franzén, Lovisa
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Massier, Lucas
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Li, Qian
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Jalkanen, Jutta
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Gao, Hui
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show more...
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Andersson, Alma
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Bhalla, Nayanika
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Thorell, Anders
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Ryden, Mikael
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Ståhl, Patrik, D ...
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Mejhert, Niklas
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Cell Metabolism
- By the university
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Royal Institute of Technology
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Karolinska Institutet