Search: onr:"swepub:oai:DiVA.org:kth-306848" >
A cell-free high th...
A cell-free high throughput assay for assessment of SARS-CoV-2 neutralizing antibodies
-
- Mravinacová, Sára (author)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
-
- Jönsson, Malin (author)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap
-
- Christ, Wanda (author)
- Karolinska Inst, Ctr Infect Med, Dept Med Huddinge, Stockholm, Sweden.
-
show more...
-
- Klingstrom, Jonas (author)
- Karolinska Institutet
-
- Yousef, Jamil (author)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
-
- Hellström, Cecilia (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Hedhammar, My, Professor, 1975- (author)
- KTH,Proteinteknologi
-
- Havervall, Sebastian (author)
- Karolinska Institutet
-
- Thalin, Charlotte (author)
- Karolinska Institutet
-
- Pin, Elisa (author)
- KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab
-
- Tegel, Hanna (author)
- KTH,Proteinteknologi,Dept Prot Sci, Div Prot Technol, Stockholm, Sweden.
-
- Nilsson, Peter (author)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
-
- Månberg, Anna, 1985- (author)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
-
- Hober, Sophia, Professor, 1965- (author)
- KTH,Science for Life Laboratory, SciLifeLab,Albanova VinnExcellence Center for Protein Technology, ProNova,Dept Prot Sci, Div Prot Technol, Stockholm, Sweden.
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
-
In: New Biotechnology. - : Elsevier BV. - 1871-6784 .- 1876-4347. ; 66, s. 46-52
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Highly accurate serological tests are key to assessing the prevalence of SARS-CoV-2 antibodies and the level of immunity in the population. This is important to predict the current and future status of the pandemic. With the recent emergence of new and more infectious SARS-CoV-2 variants, assays allowing for high throughput analysis of antibodies able to neutralize SARS-CoV-2 become even more important. Here, we report the development and validation of a robust, high throughput method, which enables the assessment of antibodies inhibiting the binding between the SARS-CoV-2 spike protein and angiotensin converting enzyme 2 (ACE2). The assay uses recombinantly produced spike-f and ACE2 and is performed in a bead array format, which allows analysis of up to 384 samples in parallel per instrument over seven hours, demanding only one hour of manual handling. The method is compared to a microneutralization assay utilising live SARS-CoV-2 and is shown to deliver highly correlating data. Further, a comparison with a serological method that measures all antibodies recognizing the spike protein shows that this type of assessment provides important insights into the neutralizing efficiency of the antibodies, especially for individuals with low antibody levels. This method can be an important and valuable tool for large-scale assessment of antibody-based neutralization, including neutralization of new spike variants that might emerge.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Medicinsk bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Keyword
- Neutralization
- Cell-free
- SARS-CoV-2
- Bead-based
- Antibodies
- Pseudoneutralization
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Mravinacová, Sár ...
-
Jönsson, Malin
-
Christ, Wanda
-
Klingstrom, Jona ...
-
Yousef, Jamil
-
Hellström, Cecil ...
-
show more...
-
Hedhammar, My, P ...
-
Havervall, Sebas ...
-
Thalin, Charlott ...
-
Pin, Elisa
-
Tegel, Hanna
-
Nilsson, Peter
-
Månberg, Anna, 1 ...
-
Hober, Sophia, P ...
-
show less...
- About the subject
-
- ENGINEERING AND TECHNOLOGY
-
ENGINEERING AND ...
-
and Industrial Biote ...
-
and Medical Biotechn ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Infectious Medic ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Immunology in th ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Medical Biotechn ...
-
and Biomedical Labor ...
- Articles in the publication
-
New Biotechnolog ...
- By the university
-
Royal Institute of Technology
-
Karolinska Institutet