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An evaluation of a ...
An evaluation of a FluoroSpot assay as a diagnostic tool to determine SARS-CoV-2 specific T cell responses
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- Mangsbo, Sara, 1981- (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för farmaceutisk biovetenskap
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- Havervall, Sebastian (author)
- Karolinska Institutet
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- Lauren, Ida (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Lindsay, Robin (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab
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- Jernbom Falk, August (author)
- KTH,Affinitets-proteomik,KTH Royal Inst Technol, Dept Prot Sci, Div Prot Technol, Stockholm, Sweden.
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- Marking, Ulrika (author)
- Karolinska Institutet
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- Lord, Martin (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Buggert, Marcus (author)
- Karolinska Institutet
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- Donnes, Pierre (author)
- SciCross AB, Skövde, Sweden.
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- Christoffersson, Gustaf (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Science for Life Laboratory, SciLifeLab
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- Nilsson, Peter (author)
- KTH,Affinitets-proteomik,KTH Royal Inst Technol, Dept Prot Sci, Div Prot Technol, Stockholm, Sweden.
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- Hober, Sophia, Professor, 1965- (author)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinteknologi,KTH Royal Inst Technol, Dept Prot Sci, Sci Life Lab, Div Affin Prote, Stockholm, Sweden.
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- Phillipson, Mia, 1973- (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Integrativ Fysiologi
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- Klingstrom, Jonas (author)
- Karolinska Institutet
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- Thalin, Charlotte (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2021-09-30
- 2021
- English.
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In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 16:9
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Abstract
Subject headings
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- Numerous assays evaluating serological and cellular responses have been developed to characterize immune responses against SARS-CoV-2. Serological assays are both cost- and time-effective compared to cellular assays, but cellular immune responses may provide a diagnostic value to determine previous SARS-CoV-2 infection in seronegative individuals. However, potential cross-reactive T cell responses stemming from prior encounters with human coronaviruses (HCoVs) may affect assay specificity. In this study, we evaluated the specificity and sensitivity of a SARS-CoV-2 IFN-gamma Release Assay (IGRA) based on the FluoroSpot method employing commercially available SARS-CoV-2-specific peptide pools, as well as an in-house designed SARS-CoV-2 peptide pool restricted to 5 amino acid stretches or less aligning with endemic HCoVs. Blood samples were obtained from healthcare workers (HCW) 5-6 months post SARS-CoV-2 spike (S) IgG and nucleocapsid (N) IgG dual seroconversion (n = 187) and HCW who had been S IgG and N IgG dual seronegative at repeated occasions, including the current sampling time point (n = 102). In addition, samples were obtained 4 to 5 months post infection from 55 polymerase chain reaction (PCR)-confirmed COVID-19 patients. Assay specificity and sensitivity were calculated with serology as a reference standard for HCW. The in-house generated peptide pool displayed a specificity of 96.1%, while the commercially available peptide pools displayed specificities of 80.4% and 85.3%, respectively. Sensitivity was higher in a cohort of previously hospitalized COVID-19 patients (96.4% and 84.0% for the commercially available peptide pools and 92.7% for the in-house generated peptide pool) compared to the HCW cohort (92.0% and 66.8% for the commercially available peptide pools and 76.0% for the in-house generated peptide pool). Based on these findings, the individual diagnostic value of T cell immune responses against SARS-CoV-2 currently appears to be limited but remain an important research tool ahead.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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Mangsbo, Sara, 1 ...
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Havervall, Sebas ...
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Lauren, Ida
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Lindsay, Robin
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Jernbom Falk, Au ...
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Marking, Ulrika
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Lord, Martin
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Buggert, Marcus
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Donnes, Pierre
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Christoffersson, ...
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Nilsson, Peter
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Hober, Sophia, P ...
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Phillipson, Mia, ...
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Klingstrom, Jona ...
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Thalin, Charlott ...
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MEDICAL AND HEAL ...
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MEDICAL AND HEAL ...
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Royal Institute of Technology
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Uppsala University
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Karolinska Institutet