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The spatial transcr...
The spatial transcriptomic landscape of the healing mouse intestine following damage
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- Parigi, Sara M. (author)
- Karolinska Inst & Univ Hosp, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Ctr Mol Med, Stockholm, Sweden.
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- Larsson, Ludvig (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Das, Srustidhar (author)
- Karolinska Institutet
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- Flores, Ricardo O. Ramirez (author)
- Heidelberg Univ, Fac Med, Heidelberg, Germany.;Heidelberg Univ Hosp, Inst Computat Biomed, Bioquant, Heidelberg, Germany.
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- Frede, Annika (author)
- Karolinska Inst & Univ Hosp, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Ctr Mol Med, Stockholm, Sweden.
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- Tripathi, Kumar P. (author)
- Karolinska Inst & Univ Hosp, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Ctr Mol Med, Stockholm, Sweden.
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- Diaz, Oscar E. (author)
- Karolinska Institutet
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- Selin, Katja (author)
- Karolinska Institutet
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- Morales, Rodrigo A. (author)
- Karolinska Institutet
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- Luo, Xinxin (author)
- Karolinska Inst & Univ Hosp, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Ctr Mol Med, Stockholm, Sweden.
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- Monasterio, Gustavo (author)
- Karolinska Inst & Univ Hosp, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Ctr Mol Med, Stockholm, Sweden.
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- Engblom, Camilla (author)
- Karolinska Institutet
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- Gagliani, Nicola (author)
- Karolinska Institutet
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- Saez-Rodriguez, Julio (author)
- Heidelberg Univ, Fac Med, Heidelberg, Germany.;Heidelberg Univ Hosp, Inst Computat Biomed, Bioquant, Heidelberg, Germany.
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- Lundeberg, Joakim (author)
- KTH,Science for Life Laboratory, SciLifeLab,Genteknologi
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- Villablanca, Eduardo J. (author)
- Karolinska Institutet
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Karolinska Inst & Univ Hosp, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden;Ctr Mol Med, Stockholm, Sweden. Genteknologi (creator_code:org_t)
- 2022-02-11
- 2022
- English.
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In: Nature Communications. - : Springer Nature. - 2041-1723. ; 13:1
- Related links:
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Abstract
Subject headings
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- The colon is comprised of specialized cells that interact with each other to function, however, the molecular regionalization of the colon is incompletely understood. Here, the authors use spatial transcriptomics to generate a publicly available resource defining the transcriptomic regionalization of the colon during steady state and mucosal healing. The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions, we demonstrate a previously unappreciated molecular regionalization of the colon, which dramatically changes during mucosal healing. Here, we identified spatially-organized transcriptional programs defining compartmentalized mucosal healing, and regions with dominant wired pathways. Furthermore, we showed that decreased p53 activation defined areas with increased presence of proliferating epithelial stem cells. Finally, we mapped transcriptomics modules associated with human diseases demonstrating the translational potential of our dataset. Overall, we provide a publicly available resource defining principles of transcriptomic regionalization of the colon during mucosal healing and a framework to develop and progress further hypotheses.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Parigi, Sara M.
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Larsson, Ludvig
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Das, Srustidhar
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Flores, Ricardo ...
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Frede, Annika
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Tripathi, Kumar ...
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show more...
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Diaz, Oscar E.
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Selin, Katja
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Morales, Rodrigo ...
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Luo, Xinxin
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Monasterio, Gust ...
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Engblom, Camilla
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Gagliani, Nicola
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Saez-Rodriguez, ...
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Lundeberg, Joaki ...
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Villablanca, Edu ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Nature Communica ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet