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Temporally Controlled Photouncaged Epidermal Growth Factor Influences Cell Fate in Hydrogels
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- Lu, Yung Hsiang (author)
- Univ Toronto, Inst Biomed Engn, Toronto, ON M5S 3G9, Canada.;Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada.
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- Baker, Alexander E. G. (author)
- Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada.;Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada.
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- Fokina, Ana (author)
- Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada.;Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada.
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- Univ Toronto, Inst Biomed Engn, Toronto, ON M5S 3G9, Canada;Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada. Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada.;Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E5, Canada. (creator_code:org_t)
- 2021-12-03
- 2022
- English.
- In: ACS Biomaterials Science & Engineering. - : American Chemical Society (ACS). - 2373-9878. ; 8:1, s. 185-195
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Hydrogels are powerful materials that more accurately mimic the cellular microenvironment over static two-dimensional culture. Photochemical strategies enable dynamic complexity to be achieved within hydrogels to better mimic the extracellular matrix; however, many photochemical systems to pattern proteins within hydrogels are complicated by long reaction times to immobilize these proteins wherein the protein can lose activity. As proof-of-concept, we demonstrate an elegant method where photocaged proteins are immobilized in hydrogels and then directly photoactivated. Specifically, we immobilized streptavidin-ortho-nitrobenzyl-modified epidermal growth factor (EGF) to cross-linked hyaluronan hydrogels and cultured two EGF-responsive cancer cells of breast and lung therein. We used light to temporally uncage and control EGF activation, thereby inducing cell death in breast cancer cells and proliferation in lung cancer cells. These results show how temporal, photochemical, protein activation influences cellular response and lays the foundation for further advances in manipulating the in vitro environment to control cell fate.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Protein Modification
- Photochemisty
- Photouncaging
- Epidermal Growth Factor
- Click Chemistry Hydrogels
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- ref (subject category)
- art (subject category)
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