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Bioorthogonally Cro...
Bioorthogonally Cross‐Linked Hyaluronan–Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication
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- Jury, Michael, 1984- (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials
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- Matthiesen, Isabelle (author)
- KTH,Mikro- och nanosystemteknik,KTH Royal Institute of Technology, Stockholm, Sweden
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- Boroojeni, Fatemeh Rasti, 1992- (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials
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- Ludwig, Saskia (author)
- KTH,Mikro- och nanosystemteknik,KTH Royal Institute of Technology, Stockholm, Sweden
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- Civitelli, Livia, 1973- (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,University of Oxford, England,Laboratory of Molecular Materials
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- Winkler, Thomas, Ph.D. (author)
- KTH,Mikro- och nanosystemteknik,Institute of Microtechnology Center of Pharmaceutical Engineering Technische Universität Braunschweig Braunschweig 38106 Germany,KTH Royal Institute of Technology, Stockholm, Sweden; Institute of Microtechnology, Center of Pharmaceutical Engineering, Technische Universität Braunschweig, Braunschweig, Germany
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- Selegård, Robert, 1981- (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials
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- Herland, Anna (author)
- Karolinska Institutet,KTH,Mikro- och nanosystemteknik,AIMES, Center for Integrated Medical and Engineering Science Department of Neuroscience Karolinska Institute Solna 171 65 Sweden;Division of Nanobiotechnology Department of Protein Science, Science for Life Laboratory KTH Royal Institute of Technology Stockholm 17165 Sweden,Division of Micro and Nanosystems, KTH Royal Institute of Technology, Stockholm, Sweden; Department of Neuroscience, Karolinska Institute, Solna, Sweden; Division of Nanobiotechnology, Department of Protein Science, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden
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- Aili, Daniel, 1977- (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials
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(creator_code:org_t)
- 2022-02-20
- 2022
- English.
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In: Advanced Healthcare Materials. - : Wiley. - 2192-2640 .- 2192-2659. ; 11:11
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Abstract
Subject headings
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- Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches. LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders. Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation. Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials. Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown. Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids. Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN. The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- 3D bioprinting; 3D cell cultures; hyaluronan; hydrogels; laminin; neural stem cells
Publication and Content Type
- ref (subject category)
- art (subject category)
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Jury, Michael, 1 ...
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Matthiesen, Isab ...
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Boroojeni, Fatem ...
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Ludwig, Saskia
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Civitelli, Livia ...
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Winkler, Thomas, ...
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Selegård, Robert ...
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Herland, Anna
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Aili, Daniel, 19 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Polymer Chemistr ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- Articles in the publication
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Advanced Healthc ...
- By the university
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Royal Institute of Technology
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Linköping University
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Karolinska Institutet