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Bioorthogonally Cro...
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Jury, Michael,1984-Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials
(author)
Bioorthogonally Cross‐Linked Hyaluronan–Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication
- Article/chapterEnglish2022
Publisher, publication year, extent ...
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2022-02-20
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Wiley,2022
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-310562
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-310562URI
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https://doi.org/10.1002/adhm.202102097DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-183407URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:148913884URI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20220405
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Funding Agencies: Swedish Foundation for Strategic Research (SFF) [FFL15-0026]; Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linköping University [2009-00971]; Carl Tryggers Foundation; Knut and Alice Wallenberg Foundation [KAW 2016.0231, 2020.0206]
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Laminins (LNs) are key components in the extracellular matrix of neuronal tissues in the developing brain and neural stem cell niches. LN-presenting hydrogels can provide a biologically relevant matrix for the 3D culture of neurons toward development of advanced tissue models and cell-based therapies for the treatment of neurological disorders. Biologically derived hydrogels are rich in fragmented LN and are poorly defined concerning composition, which hampers clinical translation. Engineered hydrogels require elaborate and often cytotoxic chemistries for cross-linking and LN conjugation and provide limited possibilities to tailor the properties of the materials. Here a modular hydrogel system for neural 3D cell cultures, based on hyaluronan and poly(ethylene glycol), that is cross-linked and functionalized with human recombinant LN-521 using bioorthogonal copper-free click chemistry, is shown. Encapsulated human neuroblastoma cells demonstrate high viability and grow into spheroids. Long-term neuroepithelial stem cells (lt-NES) cultured in the hydrogels can undergo spontaneous differentiation to neural fate and demonstrate significantly higher viability than cells cultured without LN. The hydrogels further support the structural integrity of 3D bioprinted structures and maintain high viability of bioprinted and syringe extruded lt-NES, which can facilitate biofabrication and development of cell-based therapies.
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Matthiesen, IsabelleKTH,Mikro- och nanosystemteknik,KTH Royal Institute of Technology, Stockholm, Sweden(Swepub:kth)u161smox
(author)
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Boroojeni, Fatemeh Rasti,1992-Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials(Swepub:liu)fatra27
(author)
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Ludwig, SaskiaKTH,Mikro- och nanosystemteknik,KTH Royal Institute of Technology, Stockholm, Sweden(Swepub:kth)u1r6f5yw
(author)
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Civitelli, Livia,1973-Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,University of Oxford, England,Laboratory of Molecular Materials(Swepub:liu)livci24
(author)
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Winkler, Thomas,Ph.D.KTH,Mikro- och nanosystemteknik,Institute of Microtechnology Center of Pharmaceutical Engineering Technische Universität Braunschweig Braunschweig 38106 Germany,KTH Royal Institute of Technology, Stockholm, Sweden; Institute of Microtechnology, Center of Pharmaceutical Engineering, Technische Universität Braunschweig, Braunschweig, Germany(Swepub:kth)u10hyrrq
(author)
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Selegård, Robert,1981-Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials(Swepub:liu)robse34
(author)
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Herland, AnnaKarolinska Institutet,KTH,Mikro- och nanosystemteknik,AIMES, Center for Integrated Medical and Engineering Science Department of Neuroscience Karolinska Institute Solna 171 65 Sweden;Division of Nanobiotechnology Department of Protein Science, Science for Life Laboratory KTH Royal Institute of Technology Stockholm 17165 Sweden,Division of Micro and Nanosystems, KTH Royal Institute of Technology, Stockholm, Sweden; Department of Neuroscience, Karolinska Institute, Solna, Sweden; Division of Nanobiotechnology, Department of Protein Science, Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden(Swepub:kth)u1koqi31
(author)
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Aili, Daniel,1977-Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten,Laboratory of Molecular Materials(Swepub:liu)danai11
(author)
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Linköpings universitetBiofysik och bioteknik
(creator_code:org_t)
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In:Advanced Healthcare Materials: Wiley11:112192-26402192-2659
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