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Immune-instructive ...
Immune-instructive copolymer scaffolds using plant-derived nanoparticles to promote bone regeneration
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- Suliman, Salwa (author)
- Univ Bergen, Fac Med, Ctr Translat Oral Res TOR, Dept Clin Dent, Arstadveien 19, N-5009 Bergen, Norway.
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- Mieszkowska, Anna (author)
- Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Microbiol, PL-31007 Krakow, Poland.
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- Folkert, Justyna (author)
- Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Microbiol, PL-31007 Krakow, Poland.
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- Rana, Neha (author)
- Univ Bergen, Fac Med, Ctr Translat Oral Res TOR, Dept Clin Dent, Arstadveien 19, N-5009 Bergen, Norway.
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- Mohamed-Ahmed, Samih (author)
- Univ Bergen, Fac Med, Ctr Translat Oral Res TOR, Dept Clin Dent, Arstadveien 19, N-5009 Bergen, Norway.
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- Fuoco, Tiziana, PhD, 1986- (author)
- KTH,Polymerteknologi
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- Finne-Wistrand, Anna, 1976- (author)
- KTH,Polymerteknologi
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- Dirscherl, Kai (author)
- Dansk Fundamental Metrol AS, Kogle 5, DK-2970 Horsholm, Denmark.
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- Jorgensen, Bodil (author)
- Univ Copenhagen, Dept Plant & Environm Sci, Sect Plant Glyco Biol, Copenhagen, Denmark.
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- Mustafa, Kamal (author)
- Univ Bergen, Fac Med, Ctr Translat Oral Res TOR, Dept Clin Dent, Arstadveien 19, N-5009 Bergen, Norway.
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- Gurzawska-Comis, Katarzyna (author)
- Univ Birmingham, Coll Med & Dent Sci, Inst Clin Sci, Dept Oral Surg, 5 Mill Pool Way, Birmingham B5 7EG, W Midlands, England.;Birmingham Community Healthcare NHS Fdn Trust, Birmingham Dent Hosp, Oral Surg Dept, Birmingham, W Midlands, England.
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Univ Bergen, Fac Med, Ctr Translat Oral Res TOR, Dept Clin Dent, Arstadveien 19, N-5009 Bergen, Norway Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Microbiol, PL-31007 Krakow, Poland. (creator_code:org_t)
- 2022-04-03
- 2022
- English.
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In: Inflammation and Regeneration. - : Springer Nature. - 1880-8190. ; 42:1
- Related links:
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https://doi.org/10.1...
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Abstract
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- Background Age-driven immune signals cause a state of chronic low-grade inflammation and in consequence affect bone healing and cause challenges for clinicians when repairing critical-sized bone defects in elderly patients. Methods Poly(l-lactide-co-e-caprolactone) (PLCA) scaffolds are functionalized with plant-derived nanoparticles from potato, rhamnogalacturonan-I (RG-I), to investigate their ability to modulate inflammation in vitro in neutrophils and macrophages at gene and protein levels. The scaffolds' early and late host response at gene, protein and histological levels is tested in vivo in a subcutaneous rat model and their potential to promote bone regeneration in an aged rodent was tested in a critical-sized calvaria bone defect. Significant differences were tested using one-way ANOVA, followed by a multiple-comparison Tukey's test with a p value <= 0.05 considered significant. Results Gene expressions revealed PLCA scaffold functionalized with plant-derived RG-I with a relatively higher amount of galactose than arabinose (potato dearabinated (PA)) to reduce the inflammatory state stimulated by bacterial LPS in neutrophils and macrophages in vitro. LPS-stimulated neutrophils show a significantly decreased intracellular accumulation of galectin-3 in the presence of PA functionalization compared to Control (unmodified PLCA scaffolds). The in vivo gene and protein expressions revealed comparable results to in vitro. The host response is modulated towards anti-inflammatory/ healing at early and late time points at gene and protein levels. A reduced foreign body reaction and fibrous capsule formation is observed when PLCA scaffolds functionalized with PA were implanted in vivo subcutaneously. PLCA scaffolds functionalized with PA modulated the cytokine and chemokine expressions in vivo during early and late inflammatory phases. PLCA scaffolds functionalized with PA implanted in calvaria defects of aged rats downregulating pro-inflammatory gene markers while promoting osteogenic markers after 2 weeks in vivo. Conclusion We have shown that PLCA scaffolds functionalized with plant-derived RG-I with a relatively higher amount of galactose play a role in the modulation of inflammatory responses both in vitro and in vivo subcutaneously and promote the initiation of bone formation in a critical-sized bone defect of an aged rodent. Our study addresses the increasing demand in bone tissue engineering for immunomodulatory 3D scaffolds that promote osteogenesis and modulate immune responses.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Keyword
- Plant-derived RG-I
- Copolymer
- Pectin
- Immunomodulation
- Inflammation
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Suliman, Salwa
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Mieszkowska, Ann ...
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Folkert, Justyna
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Rana, Neha
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Mohamed-Ahmed, S ...
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Fuoco, Tiziana, ...
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Finne-Wistrand, ...
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Dirscherl, Kai
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Jorgensen, Bodil
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Mustafa, Kamal
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Gurzawska-Comis, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomaterials Sci ...
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Inflammation and ...
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Royal Institute of Technology