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- van der Burg, D.Kantisto BV, Callenburglaan 22, NL-3742 MV Baarn, Netherlands.;TU Braunschweig, Inst Med & Pharmaceut Chem, Braunschweig, Germany. (author)
Method development for mono- and disaccharides monitoring in cell culture medium by capillary and microchip electrophoresis
- Article/chapterEnglish2022
Publisher, publication year, extent ...
- 2021-10-12
- Wiley,2022
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-312321
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-312321URI
- https://doi.org/10.1002/elps.202100213DOI
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-478345URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- QC 20220523
- The rapidly growing, competitive biopharmaceutical market requires tight bioprocess monitoring. An integrated, automated platform for the routine online/at-line monitoring of key factors in the cell culture medium could greatly improve process monitoring. Mono- and disaccharides, as the main energy and carbon source, are one of these key factors. A CE-LIF method was developed for the analysis of several mono- and disaccharides, considering requirements and restrictions for analysis in an integrated, automated monitoring platform, such as the possibility for miniaturization to microchip electrophoresis. Analysis was performed after fluorescent derivatization with 8-aminopyrene-1,3,6-trisulfonic acid. The derivatisation reaction and the separation BGE were optimized using design of experiments. The developed method is applicable to the complex matrix of cell culture medium and proved transferable to microchip electrophoresis.
Subject headings and genre
- TEKNIK OCH TEKNOLOGIER Maskinteknik Energiteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Mechanical Engineering Energy Engineering hsv//eng
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- CZE
- LIF-derivatization
- Microchip electrophoresis
- Process monitoring
- Saccharides
Added entries (persons, corporate bodies, meetings, titles ...)
- Josefsson, LeilaKTH,Tillämpad fysikalisk kemi,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, Stockholm, Sweden.(Swepub:kth)u1h6guji (author)
- Mikkonen, SaaraKTH,Tillämpad fysikalisk kemi,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, Stockholm, Sweden.(Swepub:kth)u1bap3az (author)
- Chotteau, Véronique,Docent,1963-KTH,Industriell bioteknologi,Proteinvetenskap,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, Stockholm, Sweden.(Swepub:kth)u1gyk88o (author)
- Emmer, ÅsaKTH,Tillämpad fysikalisk kemi,KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Dept Chem, Stockholm, Sweden.(Swepub:kth)u1czoysx (author)
- Wätzig, H.TU Braunschweig, Inst Med & Pharmaceut Chem, Braunschweig, Germany. (author)
- Sänger van de Griend, Catharina,DrUppsala universitet,Analytisk farmaceutisk kemi,Kantisto BV, Callenburglaan 22, NL-3742 MV Baarn, Netherlands.(Swepub:uu)catsa811 (author)
- Kantisto BV, Callenburglaan 22, NL-3742 MV Baarn, Netherlands.;TU Braunschweig, Inst Med & Pharmaceut Chem, Braunschweig, Germany.Tillämpad fysikalisk kemi (creator_code:org_t)
Related titles
- In:Electrophoresis: Wiley43:9-10, s. 922-9290173-08351522-2683
Internet link
- https://doi.org/10.1002/elps.202100213Fulltext
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/elps.202100213
- https://uu.diva-portal.org/smash/get/diva2:1675571/FULLTEXT01.pdffulltext:print
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-312321
- https://doi.org/10.1002/elps.202100213
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-478345
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