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- Archer, AmenaKarolinska Institutet,KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden (author)
Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells.
- Article/chapterEnglish2022
Publisher, publication year, extent ...
- 2022-02-04
- New York, NY :Springer Nature,2022
- printrdacarrier
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- LIBRIS-ID:oai:DiVA.org:kth-312637
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-312637URI
- https://doi.org/10.1007/978-1-0716-1920-9_18DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:235119673URI
Supplementary language notes
- Language:English
- Summary in:English
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Notes
- QC 20220601
- MicroRNAs play critical roles through their impact on posttranscriptional gene regulation. In cancer, they can act as oncogenes or tumor suppressors and can also function as biomarkers. Here, we describe a method for robust characterization of estrogen-regulated microRNA profiles. The activity of estrogen is mediated by two nuclear receptors, estrogen receptor alpha and estrogen receptor beta, and a transmembrane G-protein coupled estrogen receptor 1. This chapter details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, different microRNA profiling approaches, and subsequent confirmations.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- Estrogen
- MicroRNA
- Microarray
- Small RNA-Seq
- qPCR
- Bioteknologi
- Biotechnology
Added entries (persons, corporate bodies, meetings, titles ...)
- Kutter, ClaudiaKarolinska Institutet (author)
- Williams, Cecilia,Professor,1969-Karolinska Institutet,KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden,Cecilia Williams(Swepub:kth)u1ygqmuy (author)
- KTHCellulär och klinisk proteomik (creator_code:org_t)
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- In:Methods in Molecular BiologyNew York, NY : Springer Nature2418, s. 313-3431064-37451940-6029
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