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Mimicking the Biolo...
Mimicking the Biology of Engineered Protein and mRNA Nanoparticle Delivery Using a Versatile Microfluidic Platform
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- Palacio-Castaneda, Valentina (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept Biochem, Med Ctr, Geert Grootepl 28, NL-6525 GA Nijmegen, Netherlands.
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- Egberink, Rik Oude (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept Biochem, Med Ctr, Geert Grootepl 28, NL-6525 GA Nijmegen, Netherlands.
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- Sait, Arbaaz (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept Biochem, Med Ctr, Geert Grootepl 28, NL-6525 GA Nijmegen, Netherlands.
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- Andree, Lea (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept DentistryRegenerat Biomat, Med Ctr, Philips Van Leydenlaan 25, NL-6525 EX Nijmegen, Netherlands.
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- Sala, Benedetta Maria (author)
- KTH,Proteinteknologi,Albanova VinnExcellence Center for Protein Technology, ProNova
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- Besheli, Negar Hassani (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept DentistryRegenerat Biomat, Med Ctr, Philips Van Leydenlaan 25, NL-6525 EX Nijmegen, Netherlands.
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- Oosterwijk, Egbert (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept Urol, Med Ctr, Geert Grootepl 26-28, NL-6525 GA Nijmegen, Netherlands.
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- Nilvebrant, Johan, 1982- (author)
- KTH,Albanova VinnExcellence Center for Protein Technology, ProNova,Proteinteknologi,AlbaNova Univ Ctr, Sch Engn Sci Chem, Royal Inst Technol, Dept Prot Sci, SE-10044 Stockholm, Sweden.
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- Leeuwenburgh, Sander C. G. (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept DentistryRegenerat Biomat, Med Ctr, Philips Van Leydenlaan 25, NL-6525 EX Nijmegen, Netherlands.
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- Brock, Roland (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept Biochem, Med Ctr, Geert Grootepl 28, NL-6525 GA Nijmegen, Netherlands.;Arabian Gulf Univ, Coll Med & Med Sci, Dept Med Biochem, Manama 329, Bahrain.
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- Verdurmen, Wouter P. R. (author)
- Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept Biochem, Med Ctr, Geert Grootepl 28, NL-6525 GA Nijmegen, Netherlands.
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Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept Biochem, Med Ctr, Geert Grootepl 28, NL-6525 GA Nijmegen, Netherlands Radboud Univ Nijmegen, Radboud Inst Mol Life Sci, Dept DentistryRegenerat Biomat, Med Ctr, Philips Van Leydenlaan 25, NL-6525 EX Nijmegen, Netherlands. (creator_code:org_t)
- 2021-11-17
- 2021
- English.
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In: Pharmaceutics. - : MDPI AG. - 1999-4923. ; 13:11, s. 1944-
- Related links:
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https://doi.org/10.3...
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Abstract
Subject headings
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- To investigate the delivery of next-generation macromolecular drugs, such as engineered proteins and mRNA-containing nanoparticles, there is an increasing push towards the use of physiologically relevant disease models that incorporate human cells and do not face ethical dilemmas associated with animal use. Here, we illustrate the versatility and ease of use of a microfluidic platform for studying drug delivery using high-resolution microscopy in 3D. Using this microfluidic platform, we successfully demonstrate the specific targeting of carbonic anhydrase IX (CAIX) on cells overexpressing the protein in a tumor-mimicking chip system using affibodies, with CAIX-negative cells and non-binding affibodies as controls. Furthermore, we demonstrate this system's feasibility for testing mRNA-containing biomaterials designed to regenerate bone defects. To this end, peptide-and lipid-based mRNA formulations were successfully mixed with colloidal gelatin in microfluidic devices, while translational activity was studied by the expression of a green fluorescent protein. This microfluidic platform enables the testing of mRNA delivery from colloidal biomaterials of relatively high densities, which represents a first important step towards a bone-on-a-chip platform. Collectively, by illustrating the ease of adaptation of our microfluidic platform towards use in distinct applications, we show that our microfluidic chip represents a powerful and flexible way to investigate drug delivery in 3D disease-mimicking culture systems that recapitulate key parameters associated with in vivo drug application.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- drug delivery
- affibody
- CAIX
- gelatin
- mRNA
- nanoparticle
- microfluidics
- nanomedicine
- biomaterial
Publication and Content Type
- ref (subject category)
- art (subject category)
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Palacio-Castaned ...
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Egberink, Rik Ou ...
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Sait, Arbaaz
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Andree, Lea
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Sala, Benedetta ...
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Besheli, Negar H ...
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Oosterwijk, Egbe ...
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Nilvebrant, Joha ...
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Leeuwenburgh, Sa ...
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Brock, Roland
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Verdurmen, Woute ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Pharmaceutics
- By the university
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Royal Institute of Technology