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Directly immersible...
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Angelopoulou, MichailiaNCSR Demokritos, Inst Nucl & Radiol Sci & Technol Energy & Safety, Aghia Paraskevi 15341, Greece.
(author)
Directly immersible silicon photonic probes : Application to rapid SARS-CoV-2 serological testing br
- Article/chapterEnglish2022
Publisher, publication year, extent ...
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Elsevier BV,2022
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LIBRIS-ID:oai:DiVA.org:kth-317028
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-317028URI
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https://doi.org/10.1016/j.bios.2022.114570DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20220906
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Silicon photonic probes based on broad-band Mach-Zehnder interferometry are explored for the first time as directly immersible immunosensors alleviating the need for microfluidics and pumps. Each probe includes two U- shaped waveguides allowing light in- and out-coupling from the same chip side through a bifurcated fiber and a mechanical coupler. At the opposite chip side, two Mach-Zehnder interferometers (MZI) are located enabling real-time monitoring of binding reactions by immersion of this chip side into a sample. The sensing arm windows of the two MZIs have different length resulting in two distinct peaks in the Fourier domain, the phase shift of which can be monitored independently through Fast Fourier Transform of the output spectrum. The photonic probes analytical potential was demonstrated through detection of antibodies against SARS-CoV-2 in human serum samples. For this, one MZI was functionalized with the Receptor Binding Domain (RBD) of SARS-CoV-2 Spike 1 protein, and the other with bovine serum albumin to serve as reference. The biofunctionalized probes were immersed for 10 min in human serum sample and then for 5 min in goat anti-human IgG Fc specific antibody solution. Using a humanized rat antibody against SARS-CoV-2 RBD, a detection limit of 20 ng/mL was determined. Analysis of human serum samples indicated that the proposed sensor discriminated completely non- infected/non-vaccinated from vaccinated individuals, and the antibodies levels determined correlated well with those determined in the same samples by ELISA. These results demonstrated the potential of the proposed sensor to serve as an efficient tool for expeditious point-of-care testing
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Makarona, EleniNCSR Demokritos, Inst Nanosci & Nanotechnol, Aghia Paraskevi 15341, Greece.
(author)
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Salapatas, AlexandrosNCSR Demokritos, Inst Nanosci & Nanotechnol, Aghia Paraskevi 15341, Greece.
(author)
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Misiakos, KonstantinosNCSR Demokritos, Inst Nanosci & Nanotechnol, Aghia Paraskevi 15341, Greece.
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Synolaki, EvgeniaBiomed Res Fdn Acad Athens, Ctr Clin Expt Surg & Translat Res, Athens 11527, Greece.
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Ioannidis, AnastasiosUniv Peloponnese, Fac Hlth Sci, Dept Nursing, Tripoli 22100, Greece.
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Chatzipanagiotou, Stylianos
(author)
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Ritvos, Mikael A.KTH,Skolan för kemi, bioteknologi och hälsa (CBH),Natl & Kapodistrian Univ Athens, Aeginit Hosp, Med Sch, Dept Med Biopathol & Clin Microbiol, Athens 11528, Greece.;Univ Helsinki, Fac Med, Dept Physiol, Helsinki 00014, Finland.;Nord SARS Response AB, S-19455 Stockholm, Sweden.(Swepub:kth)u188rdhl
(author)
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Pasternack, ArjaNatl & Kapodistrian Univ Athens, Aeginit Hosp, Med Sch, Dept Med Biopathol & Clin Microbiol, Athens 11528, Greece.
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Ritvos, OlliNatl & Kapodistrian Univ Athens, Aeginit Hosp, Med Sch, Dept Med Biopathol & Clin Microbiol, Athens 11528, Greece.
(author)
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Petrou, Panagiota S.NCSR Demokritos, Inst Nucl & Radiol Sci & Technol Energy & Safety, Aghia Paraskevi 15341, Greece.;NCSR Demokritos, INRASTES, Res Immunoassays Immunosensors Lab, Aghia Paraskevi 15341, Greece.
(author)
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Kakabakos, Sotirios E.
(author)
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NCSR Demokritos, Inst Nucl & Radiol Sci & Technol Energy & Safety, Aghia Paraskevi 15341, Greece.NCSR Demokritos, Inst Nanosci & Nanotechnol, Aghia Paraskevi 15341, Greece.
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In:Biosensors & bioelectronics: Elsevier BV2150956-56631873-4235
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