A novel nano delivery system targeting different stages of osteoclasts

• Osteoclast (OC) abnormalities represent osteoporosis's critical mechanism (OP). OCs undergo multiple processes that range from monocytic to functional. Different drugs target OCs at different developmental stages; however, almost no Suitable drug-targeted delivery systems exist. Therefore, we designed two dual-targeting nanoparticles to target OCs at different functional stages. Using the calcitonin gene-related peptide receptor (CGRPR), which OC precursors highly express, and specific TRAPpeptides screened in the bone resorption lacuna, where mature OCs function, respectively, two types of dual-targeted nanoparticles were constructed. Afterwards, nanoparticles were grafted with hyaluronic acid (HA), which specifically binds to CD44 on the surface of the OCs. In vivo and in vitro experiments show that both nanoparticles have noticeable targeting effects on OCs. This suggests that dual-targeting nanoparticles designed for different functional periods of OC can be well targeted to the corresponding OC, and further promote the more precise delivery of drugs used to treat OP. 

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine (hsv//eng)

Keyword

Bone

Controlled drug delivery

Hyaluronic acid

Targeted drug delivery

Calcitonin gene-related peptides

Developmental stage

Different stages

Drug targets

Functional stages

Multiple process

Nano-delivery systems

Osteoclast precursor

Peptide receptor

Targeted delivery systems

Nanoparticles

acid phosphatase tartrate resistant isoenzyme

alkaline phosphatase

calcitonin

calcitonin gene related peptide

calcitonin gene related peptide receptor

calcitonin receptor like receptor

cathepsin K

colony stimulating factor 1

Hermes antigen

hyaluronic acid binding protein

layilin

membrane protein

nanoparticle

osteoclast differentiation factor

rhodamine 6G

scleroprotein

toll like receptor 4

unclassified drug

animal cell

animal experiment

animal tissue

Article

bone tissue

controlled study

drug delivery system

in vitro study

in vivo study

mouse

nanotechnology

newborn

nonhuman

osteoclast

osteolysis

osteoporosis

protein expression

human

metabolism

monocyte

Bone Resorption

Humans

Monocytes

Nanoparticle Drug Delivery System

Osteoclasts

Publication and Content Type

ref (subject category)

art (subject category)