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Structural Properties Dictating Selective Optotracer Detection of Staphylococcus aureus

Butina, Karen (author)
KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
Lantz, Linda, 1985- (author)
Linköpings universitet,Kemi,Tekniska fakulteten
Choong, Ferdinand X. (author)
Karolinska Institutet,KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
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Tomac, Ana (author)
KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
Shirani, Hamid, 1967- (author)
Linköpings universitet,Kemi,Tekniska fakulteten
Löffler, Susanne (author)
Karolinska Institutet
Nilsson, Peter, 1970- (author)
Linköpings universitet,Kemi,Tekniska fakulteten,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden
Richter-Dahlfors, Agneta (author)
Karolinska Institutet,KTH,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES,Department of Neuroscience, Karolinska Institutet, Solnavägen 9, Stockholm, 171 77, Sweden,Karolinska Inst, Sweden; KTH Royal Inst Technol, Sweden; Karolinska Inst, Sweden
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 (creator_code:org_t)
2022-04
2022
English.
In: ChemBioChem. - : Wiley. - 1439-4227 .- 1439-7633. ; 23:11
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Optotracers are conformation-sensitive fluorescent tracer molecules that detect peptide- and carbohydrate-based biopolymers. Their binding to bacterial cell walls allows selective detection and visualisation of Staphylococcus aureus (S. aureus). Here, we investigated the structural properties providing optimal detection of S. aureus. We quantified spectral shifts and fluorescence intensity in mixes of bacteria and optotracers, using automatic peak analysis, cross-correlation, and area-under-curve analysis. We found that the length of the conjugated backbone and the number of charged groups, but not their distribution, are important factors for selective detection of S. aureus. The photophysical properties of optotracers were greatly improved by incorporating a donor-acceptor-donor (D-A-D)-type motif in the conjugated backbone. With significantly reduced background and binding-induced on-switch of fluorescence, these optotracers enabled real-time recordings of S. aureus growth. Collectively, this demonstrates that chemical structure and photophysics are key tunable characteristics in the development of optotracers for selective detection of bacterial species. 

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Keyword

bacterial detection
fluorescence microscopy
fluorescence spectroscopy
optotracers
real-time monitoring
adult
area under the curve
article
bacterium detection
conformation
cross correlation
fluorescence intensity
nonhuman
quantitative analysis
spectrofluorometry
Staphylococcus aureus
bacterium
fluorescence resonance energy transfer
Bacteria

Publication and Content Type

ref (subject category)
art (subject category)

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