SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:kth-323500"
 

Search: onr:"swepub:oai:DiVA.org:kth-323500" > The cellulose synth...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

The cellulose synthase-like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley

Lou, H. (author)
Tucker, M. R. (author)
Shirley, N. J. (author)
show more...
Lahnstein, J. (author)
Yang, X. (author)
Ma, C. (author)
Schwerdt, J. (author)
Fusi, R. (author)
Burton, R. A. (author)
Band, L. R. (author)
Bennett, M. J. (author)
Bulone, Vincent (author)
KTH,Glykovetenskap,School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, 5064 Australia ARC Centre of Excellence in Plant Cell Walls, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, 5064 Australia Adelaide Glycomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Urrbrae, South Australia, 5064 Australia
show less...
 (creator_code:org_t)
2022-05-04
2022
English.
In: The Plant Journal. - : Wiley. - 0960-7412 .- 1365-313X. ; 110:6, s. 1681-1699
Related links:
https://doi.org/10.1...
show more...
https://urn.kb.se/re...
https://doi.org/10.1...
show less...
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • The barley cellulose synthase-like F (CslF) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase (CesA) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, mannan and cellulose biosynthesis, and are found in both monocots and eudicots, CslF genes are specific to the Poaceae. Recently the barley CslF3 (HvCslF3) gene was shown to be involved in the synthesis of a novel (1,4)-β-linked glucoxylan, but it remains unclear whether this gene contributes to plant growth and development. Here, expression profiling using qRT-PCR and mRNA in situ hybridization revealed that HvCslF3 accumulates in the root elongation zone. Silencing HvCslF3 by RNAi was accompanied by slower root growth, linked with a shorter elongation zone and a significant reduction in root system size. Polymer profiling of the RNAi lines revealed a significant reduction in (1,4)-β-linked glucoxylan levels. Remarkably, the heterologous expression of HvCslF3 in wild-type (Col-0) and root hair-deficient Arabidopsis mutants (csld3 and csld5) complemented the csld5 mutant phenotype, in addition to altering epidermal cell fate. Our results reveal a key role for HvCslF3 during barley root development and suggest that members of the CslD and CslF gene families have similar functions during root growth regulation. 

Subject headings

NATURVETENSKAP  -- Biologi -- Botanik (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Botany (hsv//eng)
LANTBRUKSVETENSKAPER  -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
AGRICULTURAL SCIENCES  -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

Arabidopsis
barley
cell wall
cellulose synthase-like gene
glucoxylan
Hordeum vulgare
Oroot development
Biochemistry
Biosynthesis
Cellulose
Cytology
Plants (botany)
Cell walls
Cell-wall-polysaccharides
Cellulose synthase
Root growth
Genes
glucosyltransferase
polysaccharide
gene expression regulation
genetics
Hordeum
metabolism
Gene Expression Regulation
Plant
Glucosyltransferases
Polysaccharides

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view