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Astrocyte 3D cultur...
Astrocyte 3D culture and bioprinting using peptide functionalized hyaluronan hydrogels
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- Matthiesen, Isabelle (author)
- KTH,Mikro- och nanosystemteknik,AstraZeneca, CVRM Safety, Clin Pharmacol & Safety Sci, R&D, Gothenburg, Sweden.,KTH Royal Inst Technol, Sweden; AstraZeneca, Sweden
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- Jury, Michael (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Rasti Boroojeni, Fatemeh (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Ludwig, Saskia (author)
- KTH,Mikro- och nanosystemteknik,KTH Royal Inst Technol, Sweden
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- Holzreuter, Muriel (author)
- Karolinska Inst, Ctr Integrated Med & Engn Sci, Dept Neurosci, AIMES, Solna, Sweden.
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- Buchmann, Sebastian (author)
- Karolinska Institutet,KTH,Mikro- och nanosystemteknik,Karolinska Inst, Ctr Integrated Med & Engn Sci, Dept Neurosci, AIMES, Solna, Sweden.;KTH Royal Inst Technol, Dept Prot Sci, Div Nanobiotechnol, Sci Life Lab, Solna, Sweden.
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- Träger, Andrea (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Selegård, Robert (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Winkler, Thomas, Ph.D. (author)
- KTH,Mikro- och nanosystemteknik,Tech Univ Carolo Wilhelmina Braunschweig, Inst Microtechnol, Braunschweig, Germany.;Tech Univ Carolo Wilhelmina Braunschweig, Ctr Pharmaceut Engn, Braunschweig, Germany.,KTH Royal Inst Technol, Sweden; Tech Univ Carolo Wilhelmina Braunschweig, Germany; Tech Univ Carolo Wilhelmina Braunschweig, Germany
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- Aili, Daniel (author)
- Linköpings universitet,Biofysik och bioteknik,Tekniska fakulteten
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- Herland, Anna (author)
- Karolinska Institutet,KTH,Mikro- och nanosystemteknik,Karolinska Inst, Ctr Integrated Med & Engn Sci, Dept Neurosci, AIMES, Solna, Sweden.;KTH Royal Inst Technol, Dept Prot Sci, Div Nanobiotechnol, Sci Life Lab, Solna, Sweden.
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(creator_code:org_t)
- 2023-01-30
- 2023
- English.
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In: Science and Technology of Advanced Materials. - : Informa UK Limited. - 1468-6996 .- 1878-5514. ; 24:1
- Related links:
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https://doi.org/10.1...
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https://liu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Astrocytes play an important role in the central nervous system, contributing to the development of and maintenance of synapses, recycling of neurotransmitters, and the integrity and function of the blood-brain barrier. Astrocytes are also linked to the pathophysiology of various neurodegenerative diseases. Astrocyte function and organization are tightly regulated by interactions mediated by the extracellular matrix (ECM). Engineered hydrogels can mimic key aspects of the ECM and can allow for systematic studies of ECM-related factors that govern astrocyte behaviour. In this study, we explore the interactions between neuroblastoma (SH-SY5Y) and glioblastoma (U87) cell lines and human fetal primary astrocytes (FPA) with a modular hyaluronan-based hydrogel system. Morphological analysis reveals that FPA have a higher degree of interactions with the hyaluronan-based gels compared to the cell lines. This interaction is enhanced by conjugation of cell-adhesion peptides (cRGD and IKVAV) to the hyaluronan backbone. These effects are retained and pronounced in 3D bioprinted structures. Bioprinted FPA using cRGD functionalized hyaluronan show extensive and defined protrusions and multiple connections between neighboring cells. Possibilities to tailor and optimize astrocyte-compatible ECM-mimicking hydrogels that can be processed by means of additive biofabrication can facilitate the development of advanced tissue and disease models of the central nervous system.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Keyword
- Astrocytes
- 3d cell culture
- bioprinting
- hyaluronan
- cRGD
- IKVAV
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Matthiesen, Isab ...
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Jury, Michael
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Rasti Boroojeni, ...
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Ludwig, Saskia
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Holzreuter, Muri ...
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Buchmann, Sebast ...
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show more...
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Träger, Andrea
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Selegård, Robert
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Winkler, Thomas, ...
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Aili, Daniel
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Herland, Anna
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Materials Chemis ...
- Articles in the publication
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Science and Tech ...
- By the university
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Royal Institute of Technology
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Linköping University
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Karolinska Institutet