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Color-Switchable Na...
Color-Switchable Nanosilicon Fluorescent Probes
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Chen, H. (author)
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Xu, J. (author)
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Wang, Y. (author)
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Wang, D. (author)
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Ferrer-Espada, R. (author)
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- Zhou, Jingjian, 1993- (author)
- KTH,Tillämpad fysik
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Pedrazo-Tardajos, A. (author)
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Yang, M. (author)
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Tan, J. -H (author)
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Yang, X. (author)
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Zhang, L. (author)
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- Sychugov, Ilya (author)
- KTH,Tillämpad fysik
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Chen, S. (author)
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Bals, S. (author)
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Paulsson, J. (author)
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Yang, Z. (author)
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(creator_code:org_t)
- 2022-09-15
- 2022
- English.
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In: ACS Nano. - : American Chemical Society (ACS). - 1936-0851 .- 1936-086X. ; 16:9, s. 15450-15459
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Fluorescent probes are vital to cell imaging by allowing specific parts of cells to be visualized and quantified. Color-switchable probes (CSPs), with tunable emission wavelength upon contact with specific targets, are particularly powerful because they not only eliminate the need to wash away all unbound probe but also allow for internal controls of probe concentrations, thereby facilitating quantification. Several such CSPs exist and have proven very useful, but not for all key cellular targets. Here we report a pioneering CSP for in situ cell imaging using aldehyde-functionalized silicon nanocrystals (SiNCs) that switch their intrinsic photoluminescence from red to blue quickly when interacting with amino acids in live cells. Though conventional probes often work better in cell-free extracts than in live cells, the SiNCs display the opposite behavior and function well and fast in universal cell lines at 37 °C while requiring much higher temperature in extracts. Furthermore, the SiNCs only disperse in cytoplasm not nucleus, and their fluorescence intensity correlated linearly with the concentration of fed amino acids. We believe these nanosilicon probes will be promising tools to visualize distribution of amino acids and potentially quantify amino acid related processes in live cells.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- bioimaging
- color-switchable probe
- nanocrystal
- photoluminescence
- silicon
- surface chemistry
- Amino acids
- Cell culture
- Cells
- Color
- Fluorescence
- Nanocrystals
- Probes
- Silicon compounds
- Amino-acids
- Bio-imaging
- Cell imaging
- Fluorescent probes
- Live cell
- Nano-silicon
- Silicon nanocrystals
- Switchable
- Tunable emissions
- aldehyde
- amino acid
- fluorescent dye
- nanoparticle
- Aldehydes
- Fluorescent Dyes
- Nanoparticles
Publication and Content Type
- ref (subject category)
- art (subject category)
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ACS Nano
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To the university's database
- By the author/editor
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Chen, H.
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Xu, J.
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Wang, Y.
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Wang, D.
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Ferrer-Espada, R ...
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Zhou, Jingjian, ...
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show more...
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Pedrazo-Tardajos ...
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Yang, M.
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Tan, J. -H
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Yang, X.
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Zhang, L.
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Sychugov, Ilya
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Chen, S.
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Bals, S.
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Paulsson, J.
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Yang, Z.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
- Articles in the publication
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ACS Nano
- By the university
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Royal Institute of Technology